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. 1976 Mar;125(3):755–761. doi: 10.1128/jb.125.3.755-761.1976

Identification of coreplicating chromosomal sectors in Bacillus subtilis by nitrosoguanidine-induced comutation.

A G Siccardi, F A Ferrari, G Mazza, A Galizzi
PMCID: PMC236145  PMID: 815245

Abstract

The simultaneous replication of four regions of dichotomously replicating chromosomes of Bacillus subtilis has been detected by means of nitrosoguanidine-induced comutation. The map distance between successive rounds of replication has been measured as one-half a replicative arm in cells growing exponentially in rich medium.

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Selected References

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  1. BARAT M., ANAGNOSTOPOULOS C., SCHNEIDER A. M. LINKAGE RELATIONSHIPS OF GENES CONTROLLING ISOLEUCINE, VALINE, AND LEUCINE BIOSYNTHESIS IN BACILLUS SUBTILIS. J Bacteriol. 1965 Aug;90:357–369. doi: 10.1128/jb.90.2.357-369.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Borenstein S., Ephrati-Elizur E. Spontaneous release of DNA in sequential genetic order by Bacillus subtilis. J Mol Biol. 1969 Oct 14;45(1):137–152. doi: 10.1016/0022-2836(69)90216-2. [DOI] [PubMed] [Google Scholar]
  3. Cerdá-Olmedo E., Hanawalt P. C., Guerola N. Mutagenesis of the replication point by nitrosoguanidine: map and pattern of replication of the Escherichia coli chromosome. J Mol Biol. 1968 May 14;33(3):705–719. doi: 10.1016/0022-2836(68)90315-x. [DOI] [PubMed] [Google Scholar]
  4. Chilton M. D., McCarthy B. J. Genetic and base sequence homologies in bacillus. Genetics. 1969 Jul;62(3):697–710. [PMC free article] [PubMed] [Google Scholar]
  5. Georgopoulos C. P. Suppressor system in Bacillus subtilis 168. J Bacteriol. 1969 Mar;97(3):1397–1402. doi: 10.1128/jb.97.3.1397-1402.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Guerola N., Ingraham J. L., Cerdá-Olmedo E. Induction of closely linked multiple mutations by nitrosoguanidine. Nat New Biol. 1971 Mar 24;230(12):122–125. doi: 10.1038/newbio230122a0. [DOI] [PubMed] [Google Scholar]
  7. Gyurasits E. B., Wake R. G. Bidirectional chromosome replication in Bacillus subtilis. J Mol Biol. 1973 Jan;73(1):55–63. doi: 10.1016/0022-2836(73)90158-7. [DOI] [PubMed] [Google Scholar]
  8. Hara H., Yoshikawa H. Asymmetric bidirectional replication of Bacillus subtilis chromosome. Nat New Biol. 1973 Aug 15;244(137):200–203. doi: 10.1038/newbio244200a0. [DOI] [PubMed] [Google Scholar]
  9. Harford N. Bidirectional chromosome replication in Bacillus subtilis 168. J Bacteriol. 1975 Mar;121(3):835–847. doi: 10.1128/jb.121.3.835-847.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hoch J. A., Barat M., Anagnostopoulos C. Transformation and transduction in recombination-defective mutants of Bacillus subtilis. J Bacteriol. 1967 Jun;93(6):1925–1937. doi: 10.1128/jb.93.6.1925-1937.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LEDERBERG J., LEDERBERG E. M. Replica plating and indirect selection of bacterial mutants. J Bacteriol. 1952 Mar;63(3):399–406. doi: 10.1128/jb.63.3.399-406.1952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lepesant-Kejzlarová J., Lepesant J. A., Walle J., Billault A., Dedonder R. Revision of the linkage map of Bacillus subtilis 168: indications for circularity of the chromosome. J Bacteriol. 1975 Mar;121(3):823–834. doi: 10.1128/jb.121.3.823-834.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lepesant-Kejzlarová J., Walle J., Billault A., Kunst F., Lepesant J. A., Dedonder R. Etablissement de la carte génétique de Bacillus subtilis: réexamen de la localisation du segment chromosomique compris entre les marqueurs sacQ36 et gtaA12. C R Acad Sci Hebd Seances Acad Sci D. 1974 Apr 1;278(14):1911–1914. [PubMed] [Google Scholar]
  14. Lepesant J. A., Kunst F., Lepesant-Kejzlarová J., Dedonder R. Chromosomal location of mutations affecting sucrose metabolism in Bacillus subtilis Marburg. Mol Gen Genet. 1972;118(2):135–160. doi: 10.1007/BF00267084. [DOI] [PubMed] [Google Scholar]
  15. O'Sullivan A., Sueoka N. Sequential replication of the Bacillus subtilis chromosome. IV. Genetic mapping by density transfer experiment. J Mol Biol. 1967 Jul 28;27(2):349–368. doi: 10.1016/0022-2836(67)90025-3. [DOI] [PubMed] [Google Scholar]
  16. Riva S., van Sluis C., Mastromei G., Attolini C., Mazza G., Polsinelli M., Falaschi A. A new mutant of Bacillus subtilis altered in the initiation of chromosome replication. Mol Gen Genet. 1975;137(3):185–202. doi: 10.1007/BF00333015. [DOI] [PubMed] [Google Scholar]
  17. Siccardi A. G., Galizzi A., Mazza G., Clivio A., Albertini A. M. Synchronous germination and outgrowth of fractionated Bacillus subtilis spores: tool for the analysis of differentiation and division of bacterial cells. J Bacteriol. 1975 Jan;121(1):13–19. doi: 10.1128/jb.121.1.13-19.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Spizizen J. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072–1078. doi: 10.1073/pnas.44.10.1072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. TAKAHASHI I. Transducing phages for Bacillus subtilis. J Gen Microbiol. 1963 May;31:211–217. doi: 10.1099/00221287-31-2-211. [DOI] [PubMed] [Google Scholar]
  20. Wake R. G. Circularity of the Bacillus subtilis chromosome and further studies on its bidirectional replication. J Mol Biol. 1973 Jul 15;77(4):569–575. doi: 10.1016/0022-2836(73)90223-4. [DOI] [PubMed] [Google Scholar]
  21. Wake R. G. Termination of Bacillus subtilis chromosome replication as visualized by autoradiography. J Mol Biol. 1974 Jun 25;86(2):223–231. doi: 10.1016/0022-2836(74)90014-x. [DOI] [PubMed] [Google Scholar]
  22. YOSHIKAWA H., SUEOKA N. Sequential replication of Bacillus subtilis chromosome. I. Comparison of marker frequencies in exponential and stationary growth phases. Proc Natl Acad Sci U S A. 1963 Apr;49:559–566. doi: 10.1073/pnas.49.4.559. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. YOSHIKAWA H., SUEOKA N. Sequential replication of the Bacillus subtilis chromosome. II. Isotopic transfer experiments. Proc Natl Acad Sci U S A. 1963 Jun;49:806–813. doi: 10.1073/pnas.49.6.806. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Yamagishi H., Takahashi I. Transducing particles of PBS 1. Virology. 1968 Dec;36(4):639–645. doi: 10.1016/0042-6822(68)90194-3. [DOI] [PubMed] [Google Scholar]

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