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. 1999 Jan;79(2):214–220. doi: 10.1038/sj.bjc.6690036

Breast cancer cell-associated endopeptidase EC 24.11 modulates proliferative response to bombesin

D M Burns 1, B Walker 2, J Gray 2, J Nelson 2
PMCID: PMC2362209  PMID: 9888460

Abstract

We have investigated the production, growth and inactivation of gastrin-releasing peptide (GRP)-like peptides in human breast cancer cell lines. Radioimmunoassay detected GRP-like immunoreactivity (GRP-LI) in T47D breast cancer cells but not in the conditioned medium, indicating rapid clearance. No GRP-LI was found in the ZR-75-1 or MDA-MB-436 cells or their conditioned medium. High-performance liquid chromatography (HPLC) analysis of the GRP-LI in the T47D cells revealed a major peak, which co-eluted with GRP18–27, and a minor more hydrophilic peak. In vitro stimulation of T47D cell growth by bombesin (BN) was enhanced to 138% of control levels (bombesin alone) by the addition of the selective endopeptidase EC 3.4.24.11 inhibitor phosphoramidon (0.1 ng ml−;1). Fluorogenic analysis using whole cells confirmed low levels of this phosphoramidon-sensitive enzyme on the T47D cells. This enzyme, previously unreported in human breast cancer cells, significantly modulates both T47D growth and its response to BN-induced growth. © 1999 Cancer Research Campaign

Keywords: enkephalinase, bombesin, gastrin-releasing peptide, breast cancer cells, neutral endopeptidase 24.11

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Selected References

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