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. 1994 Jan;68(1):535–540. doi: 10.1128/jvi.68.1.535-540.1994

Restriction and enhancement of human immunodeficiency virus type 1 replication by modulation of intracellular deoxynucleoside triphosphate pools.

A Meyerhans 1, J P Vartanian 1, C Hultgren 1, U Plikat 1, A Karlsson 1, L Wang 1, S Eriksson 1, S Wain-Hobson 1
PMCID: PMC236318  PMID: 8254768

Abstract

Human immunodeficiency virus type 1 (HIV-1) replication is shown to be sensitive to the intracellular concentration of deoxynucleoside triphosphate substrates. Addition of thymidine to established cell lines resulted in a dramatic reduction of virus production. The effect could be substantially alleviated by addition of deoxycytidine, which, alone, enhanced viral titers by a factor of 2 to 3. Hydroxyurea treatment abolished HIV-1 replication in peripheral blood mononuclear cells and could be reversed by deoxyadenosine. These data show that HIV-1 replication occurs under suboptimal DNA precursor conditions.

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Selected References

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