Abstract
The identification of influenza virus type has traditionally been based on the characterization of internal nucleoprotein (NP) antigens by the complement fixation (CF) test. Because this test is complex and time-consuming, it is used only infrequently. In this report we describe a double immunodiffusion (DID) test, which is proposed as a replacement for the CF test for the typing of influenza viruses. The DID test is simple and requires only the allantoic fluid content of a single infected embryonated egg. Virus from the infected fluid is precipitated with mild acid and disrupted by detergent. Anti-NP or anti-matrix protein (MP) antibody is equally effective in most instances for typing influenza isolates by this test. The latter is preferred, however, since it seemed to be slightly more sensitive than anti-NP antibody.
Full text
PDFImages in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Hierholzer J. C., Suggs M. T., Hall E. C. Standardized viral hemagglutination and hemagglutination-inhibition tests. II. Description and statistical evaluation. Appl Microbiol. 1969 Nov;18(5):824–833. doi: 10.1128/am.18.5.824-833.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schild G. C., Pereira H. G. Characterization of the ribonucleoprotein and neuraminidase of influenza A viruses by immunodiffusion. J Gen Virol. 1969 Apr;4(3):355–363. doi: 10.1099/0022-1317-4-3-355. [DOI] [PubMed] [Google Scholar]
- Skehel J. J., Schild G. C. The polypeptide composition of influenza A viruses. Virology. 1971 May;44(2):396–408. doi: 10.1016/0042-6822(71)90270-4. [DOI] [PubMed] [Google Scholar]
- Styk B., Hána L. Immunodiffusion studies on the reaction of influenza virus with specific antibody and nonspecific serum beta-inhibitor. Acta Virol. 1966 Jul;10(4):281–290. [PubMed] [Google Scholar]