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. 1976;54(2):129–139.

The enzyme-linked immunosorbent assay (ELISA)*

PMCID: PMC2366430  PMID: 798633

Abstract

The enzyme-linked immunosorbent assay “ELISA” developed in recent years represents a significant addition to existing serological tools. Encouraging preliminary results obtained through its application to a number of parasitic diseases during the last two years indicate the value of further investigations and trials which will permit a true evaluation.

Although the technique is easy to perform and quite sensitive, there are certain problems to be solved before it becomes widely usable. In the present Memorandum the technical details are given and the advantages and shortcomings of the procedure are discussed. Present applications and future prospects are reviewed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bartlett A., Bidwell D. E., Voller A. Preliminary studies on the application of enzyme immunoassay in the detection of antibodies in onchocerciasis. Tropenmed Parasitol. 1975 Sep;26(3):370–374. [PubMed] [Google Scholar]
  2. Bout D., Dugimont J. C., Farag H., Capron A. Le diagnostic immunoenzymologique des affections parasitaires. II - Immunoenzymologie quantitative sur lame. Lille Med. 1975 Jun-Jul;20(6):561–566. [PubMed] [Google Scholar]
  3. Deelder A. M., Ploem J. S. An immunofluorescence reaction for Schistosoma manosoni using the defined antigen substrate spheres (DASS) system. J Immunol Methods. 1974 Mar;4(2):239–251. doi: 10.1016/0022-1759(74)90067-2. [DOI] [PubMed] [Google Scholar]
  4. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry. 1971 Sep;8(9):871–874. doi: 10.1016/0019-2791(71)90454-x. [DOI] [PubMed] [Google Scholar]
  5. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol. 1972 Jul;109(1):129–135. [PubMed] [Google Scholar]
  6. Ruitenberg E. J., Ljungström I., Steerenberg P. A., Buys J. Application of immunofluorescence and immunoenzyme methods in the serodiagnosis of Trichinella spiralis infection. Ann N Y Acad Sci. 1975 Jun 30;254:296–303. doi: 10.1111/j.1749-6632.1975.tb29179.x. [DOI] [PubMed] [Google Scholar]
  7. Ruitenberg E. J., Steerenberg P. A., Brosi B. J., Buys J. ELISA (enzyme-linked immunosorbent assay) as preventive and repressive control method for the detection of Trichinella spiralis infections in slaughter pigs. Wiad Parazytol. 1975;21(4-5):747–751. [PubMed] [Google Scholar]
  8. Ruitenberg E. J., Steerenberg P. A., Brosi B. J., Buys J. Reliability of the enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of Trichinella spiralis infections in conventionally raised pigs. J Immunol Methods. 1976;10(1):67–83. doi: 10.1016/0022-1759(76)90008-9. [DOI] [PubMed] [Google Scholar]
  9. Ruitenberg E. J., Steerenberg P. A., Brosi B. J., Buys J. Serodiagnosis of Trichinella spiralis infections in pigs by enzyme-linked immunosorbent assays. Bull World Health Organ. 1974;51(1):108–109. [PMC free article] [PubMed] [Google Scholar]
  10. Van Weemen B. K., Schuurs A. H.W.M. Immunoassay using antigen-enzyme conjugates. FEBS Lett. 1971 Jun 24;15(3):232–236. doi: 10.1016/0014-5793(71)80319-8. [DOI] [PubMed] [Google Scholar]
  11. Voller A., Bidwell D. E., Bartlett A., Fleck D. G., Perkins M., Oladehin B. A microplate enzyme-immunoassay for toxoplasma antibody. J Clin Pathol. 1976 Feb;29(2):150–153. doi: 10.1136/jcp.29.2.150. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Voller A., Bidwell D., Bartlett A. A serological study on human Trypanosoma rhodesiense infections using a micro-scale enzyme linked immunosorbent assay. Tropenmed Parasitol. 1975 Jun;26(2):247–251. [PubMed] [Google Scholar]
  13. Voller A., Bidwell D., Huldt G., Engvall E. A microplate method of enzyme-linked immunosorbent assay and its application to malaria. Bull World Health Organ. 1974;51(2):209–211. [PMC free article] [PubMed] [Google Scholar]
  14. Voller A., Draper C., Bidwell D. E., Bartlett A. Microplate enzyme-linked immunosorbent assay for chagas' disease. Lancet. 1975 Feb 22;1(7904):426–428. doi: 10.1016/s0140-6736(75)91492-0. [DOI] [PubMed] [Google Scholar]
  15. Voller A., Huldt G., Thors C., Engvall E. New serological test for malaria antibodies. Br Med J. 1975 Mar 22;1(5959):659–661. doi: 10.1136/bmj.1.5959.659. [DOI] [PMC free article] [PubMed] [Google Scholar]

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