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. 1994 Apr;68(4):2224–2229. doi: 10.1128/jvi.68.4.2224-2229.1994

Human immunodeficiency virus type 1 Rev-responsive element RNA binds to host cell-specific proteins.

R R Shukla 1, P L Kimmel 1, A Kumar 1
PMCID: PMC236698  PMID: 8139007

Abstract

RNase protection-gel retention studies show human host cell-specific ribonucleoprotein complexes with human immunodeficiency virus type 1 Rev-responsive element (RRE) RNA. Nuclear proteins from rodent or murine cells appear to lack the ability to form these complexes. Human-mouse somatic cell hybrids retaining a single human chromosome, either 6 or 12, form the RRE-nuclear-protein complexes. One of the complexes requires the entire RRE RNA, while the other needs RRE RNA stem-loops 1 and 2 only. Two major proteins with molecular masses of 120 and 62 kDa specifically bind to RRE RNA. Rodent cells (CHO) either lack or contain small amounts of these RRE-binding proteins.

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