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. 1994 Sep;68(9):6052–6056. doi: 10.1128/jvi.68.9.6052-6056.1994

Ordered assembly of a functional preinitiation transcription complex, containing vaccinia virus early transcription factor and RNA polymerase, on an immobilized template.

C J Baldick Jr 1, M C Cassetti 1, N Harris 1, B Moss 1
PMCID: PMC237011  PMID: 8057480

Abstract

A functional preinitiation transcription complex was formed by incubating vaccinia virus early transcription factor VETF and RAP94+ RNA polymerase with an early promoter template immobilized on paramagnetic particles. A preferred order of assembly, VETF followed by RNA polymerase, was demonstrated by stepwise addition experiments. ATP was unnecessary for preinitiation transcription complex formation, but divalent cations were required specifically for the association of RNA polymerase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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