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. 1993 Jun;67(6):3611–3614. doi: 10.1128/jvi.67.6.3611-3614.1993

Subgenomic RNA sequence of human astrovirus supports classification of Astroviridae as a new family of RNA viruses.

S S Monroe 1, B Jiang 1, S E Stine 1, M Koopmans 1, R I Glass 1
PMCID: PMC237710  PMID: 8497068

Abstract

We report the sequence of the subgenomic RNA of human astrovirus serotype 2. This 2,484-nucleotide RNA contains a single open reading frame, which encodes a protein with a predicted molecular mass of 88 kDa. We propose that this protein is the 90-kDa capsid precursor observed in infected cells. The deduced protein sequence does not contain conserved amino acid patterns reported for the capsid proteins of picornaviruses or caliciviruses, consistent with the classification of astroviruses as a new family of RNA viruses, designated Astroviridae.

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Selected References

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  1. Appleton H., Higgins P. G. Letter: Viruses and gastroenteritis in infants. Lancet. 1975 Jun 7;1(7919):1297–1297. doi: 10.1016/s0140-6736(75)92581-7. [DOI] [PubMed] [Google Scholar]
  2. Cruz J. R., Bartlett A. V., Herrmann J. E., Cáceres P., Blacklow N. R., Cano F. Astrovirus-associated diarrhea among Guatemalan ambulatory rural children. J Clin Microbiol. 1992 May;30(5):1140–1144. doi: 10.1128/jcm.30.5.1140-1144.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Devereux J., Haeberli P., Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. doi: 10.1093/nar/12.1part1.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Herrmann J. E., Nowak N. A., Perron-Henry D. M., Hudson R. W., Cubitt W. D., Blacklow N. R. Diagnosis of astrovirus gastroenteritis by antigen detection with monoclonal antibodies. J Infect Dis. 1990 Feb;161(2):226–229. doi: 10.1093/infdis/161.2.226. [DOI] [PubMed] [Google Scholar]
  5. Herrmann J. E., Taylor D. N., Echeverria P., Blacklow N. R. Astroviruses as a cause of gastroenteritis in children. N Engl J Med. 1991 Jun 20;324(25):1757–1760. doi: 10.1056/NEJM199106203242501. [DOI] [PubMed] [Google Scholar]
  6. Kurtz J. B., Lee T. W. Human astrovirus serotypes. Lancet. 1984 Dec 15;2(8416):1405–1405. doi: 10.1016/s0140-6736(84)92101-9. [DOI] [PubMed] [Google Scholar]
  7. Lew J. F., Moe C. L., Monroe S. S., Allen J. R., Harrison B. M., Forrester B. D., Stine S. E., Woods P. A., Hierholzer J. C., Herrmann J. E. Astrovirus and adenovirus associated with diarrhea in children in day care settings. J Infect Dis. 1991 Oct;164(4):673–678. doi: 10.1093/infdis/164.4.673. [DOI] [PubMed] [Google Scholar]
  8. Madeley C. R., Cosgrove B. P. Letter: 28 nm particles in faeces in infantile gastroenteritis. Lancet. 1975 Sep 6;2(7932):451–452. doi: 10.1016/s0140-6736(75)90858-2. [DOI] [PubMed] [Google Scholar]
  9. Matsui S. M., Kim J. P., Greenberg H. B., Young L. M., Smith L. S., Lewis T. L., Herrmann J. E., Blacklow N. R., Dupuis K., Reyes G. R. Cloning and characterization of human astrovirus immunoreactive epitopes. J Virol. 1993 Mar;67(3):1712–1715. doi: 10.1128/jvi.67.3.1712-1715.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Moe C. L., Allen J. R., Monroe S. S., Gary H. E., Jr, Humphrey C. D., Herrmann J. E., Blacklow N. R., Carcamo C., Koch M., Kim K. H. Detection of astrovirus in pediatric stool samples by immunoassay and RNA probe. J Clin Microbiol. 1991 Nov;29(11):2390–2395. doi: 10.1128/jcm.29.11.2390-2395.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Monroe S. S., Glass R. I., Noah N., Flewett T. H., Caul E. O., Ashton C. I., Curry A., Field A. M., Madeley R., Pead P. J. Electron microscopic reporting of gastrointestinal viruses in the United Kingdom, 1985-1987. J Med Virol. 1991 Mar;33(3):193–198. doi: 10.1002/jmv.1890330310. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Monroe S. S., Stine S. E., Gorelkin L., Herrmann J. E., Blacklow N. R., Glass R. I. Temporal synthesis of proteins and RNAs during human astrovirus infection of cultured cells. J Virol. 1991 Feb;65(2):641–648. doi: 10.1128/jvi.65.2.641-648.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Needleman S. B., Wunsch C. D. A general method applicable to the search for similarities in the amino acid sequence of two proteins. J Mol Biol. 1970 Mar;48(3):443–453. doi: 10.1016/0022-2836(70)90057-4. [DOI] [PubMed] [Google Scholar]
  14. Neill J. D. Nucleotide sequence of the capsid protein gene of two serotypes of San Miguel sea lion virus: identification of conserved and non-conserved amino acid sequences among calicivirus capsid proteins. Virus Res. 1992 Jul;24(2):211–222. doi: 10.1016/0168-1702(92)90008-w. [DOI] [PubMed] [Google Scholar]
  15. Tohya Y., Taniguchi Y., Takahashi E., Utagawa E., Takeda N., Miyamura K., Yamazaki S., Mikami T. Sequence analysis of the 3'-end of feline calicivirus genome. Virology. 1991 Aug;183(2):810–814. doi: 10.1016/0042-6822(91)91016-A. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Willcocks M. M., Carter M. J., Silcock J. G., Madeley C. R. A dot-blot hybridization procedure for the detection of astrovirus in stool samples. Epidemiol Infect. 1991 Oct;107(2):405–410. doi: 10.1017/s0950268800049049. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Willcocks M. M., Carter M. J. The 3' terminal sequence of a human astrovirus. Arch Virol. 1992;124(3-4):279–289. doi: 10.1007/BF01309809. [DOI] [PubMed] [Google Scholar]
  18. Zuker M., Stiegler P. Optimal computer folding of large RNA sequences using thermodynamics and auxiliary information. Nucleic Acids Res. 1981 Jan 10;9(1):133–148. doi: 10.1093/nar/9.1.133. [DOI] [PMC free article] [PubMed] [Google Scholar]

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