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. 1993 Oct;67(10):5867–5872. doi: 10.1128/jvi.67.10.5867-5872.1993

Sequence and temporal appearance of the early transcribed baculovirus gene HE65.

D Becker 1, D Knebel-Mörsdorf 1
PMCID: PMC238005  PMID: 8371346

Abstract

We have identified the early transcribed HE65 gene by screening a cDNA library from polyadenylated RNA which was isolated at 1 h after infection of Spodoptera frugiperda cells with Autographa californica nuclear polyhedrosis virus (AcNPV). Nucleotide sequencing analysis of the HE65-specific cDNA clone reveals one open reading frame of 1,662 nucleotides from which a protein of 65 kDa in size can be predicted. The HE65 gene is located downstream of the late transcribed p80 gene and upstream of the homologous region hr4left, which overlaps the 5' sequences of the HE65 gene. An HE65-specific transcript of about 1,800 nucleotides is detectable 2 h postinfection and remains stable during the late phases of infection. RNase protection and primer extension analyses demonstrate that transcripts from the early start site of HE65 continue to accumulate from 2 to 48 h postinfection, even in the presence of aphidicolin. Furthermore, transcriptional analysis of the HE65 gene indicates a lower intensity of early transcription in comparison with the very early transcribed genes IEN, PE38, and ME53.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blissard G. W., Rohrmann G. F. Baculovirus diversity and molecular biology. Annu Rev Entomol. 1990;35:127–155. doi: 10.1146/annurev.en.35.010190.001015. [DOI] [PubMed] [Google Scholar]
  2. Carson D. D., Guarino L. A., Summers M. D. Functional mapping of an AcNPV immediately early gene which augments expression of the IE-1 trans-activated 39K gene. Virology. 1988 Feb;162(2):444–451. doi: 10.1016/0042-6822(88)90485-0. [DOI] [PubMed] [Google Scholar]
  3. Carson D. D., Summers M. D., Guarino L. A. Molecular analysis of a baculovirus regulatory gene. Virology. 1991 May;182(1):279–286. doi: 10.1016/0042-6822(91)90671-w. [DOI] [PubMed] [Google Scholar]
  4. Carson D. D., Summers M. D., Guarino L. A. Transient expression of the Autographa californica nuclear polyhedrosis virus immediate-early gene, IE-N, is regulated by three viral elements. J Virol. 1991 Feb;65(2):945–951. doi: 10.1128/jvi.65.2.945-951.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chisholm G. E., Henner D. J. Multiple early transcripts and splicing of the Autographa californica nuclear polyhedrosis virus IE-1 gene. J Virol. 1988 Sep;62(9):3193–3200. doi: 10.1128/jvi.62.9.3193-3200.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cochran M. A., Faulkner P. Location of Homologous DNA Sequences Interspersed at Five Regions in the Baculovirus AcMNPV Genome. J Virol. 1983 Mar;45(3):961–970. doi: 10.1128/jvi.45.3.961-970.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Crawford A. M., Miller L. K. Characterization of an early gene accelerating expression of late genes of the baculovirus Autographa californica nuclear polyhedrosis virus. J Virol. 1988 Aug;62(8):2773–2781. doi: 10.1128/jvi.62.8.2773-2781.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Friesen P. D., Miller L. K. Divergent transcription of early 35- and 94-kilodalton protein genes encoded by the HindIII K genome fragment of the baculovirus Autographa californica nuclear polyhedrosis virus. J Virol. 1987 Jul;61(7):2264–2272. doi: 10.1128/jvi.61.7.2264-2272.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fuchs L. Y., Woods M. S., Weaver R. F. Viral Transcription During Autographa californica Nuclear Polyhedrosis Virus Infection: a Novel RNA Polymerase Induced in Infected Spodoptera frugiperda Cells. J Virol. 1983 Dec;48(3):641–646. doi: 10.1128/jvi.48.3.641-646.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Guarino L. A., Gonzalez M. A., Summers M. D. Complete Sequence and Enhancer Function of the Homologous DNA Regions of Autographa californica Nuclear Polyhedrosis Virus. J Virol. 1986 Oct;60(1):224–229. doi: 10.1128/jvi.60.1.224-229.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Guarino L. A., Smith M. W. Nucleotide sequence and characterization of the 39K gene region of Autographa californica nuclear polyhedrosis virus. Virology. 1990 Nov;179(1):1–8. doi: 10.1016/0042-6822(90)90266-t. [DOI] [PubMed] [Google Scholar]
  12. Guarino L. A., Smith M. Regulation of delayed-early gene transcription by dual TATA boxes. J Virol. 1992 Jun;66(6):3733–3739. doi: 10.1128/jvi.66.6.3733-3739.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Guarino L. A., Summers M. D. Functional mapping of a trans-activating gene required for expression of a baculovirus delayed-early gene. J Virol. 1986 Feb;57(2):563–571. doi: 10.1128/jvi.57.2.563-571.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Guarino L. A., Summers M. D. Interspersed Homologous DNA of Autographa californica Nuclear Polyhedrosis Virus Enhances Delayed-Early Gene Expression. J Virol. 1986 Oct;60(1):215–223. doi: 10.1128/jvi.60.1.215-223.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Guarino L. A., Summers M. D. Nucleotide sequence and temporal expression of a baculovirus regulatory gene. J Virol. 1987 Jul;61(7):2091–2099. doi: 10.1128/jvi.61.7.2091-2099.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Knebel-Mörsdorf D., Kremer A., Jahnel F. Baculovirus gene ME53, which contains a putative zinc finger motif, is one of the major early-transcribed genes. J Virol. 1993 Feb;67(2):753–758. doi: 10.1128/jvi.67.2.753-758.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kool M., van den Berg P. M., Tramper J., Goldbach R. W., Vlak J. M. Location of two putative origins of DNA replication of Autographa californica nuclear polyhedrosis virus. Virology. 1993 Jan;192(1):94–101. doi: 10.1006/viro.1993.1011. [DOI] [PubMed] [Google Scholar]
  18. Krappa R., Behn-Krappa A., Jahnel F., Doerfler W., Knebel-Mörsdorf D. Differential factor binding at the promoter of early baculovirus gene PE38 during viral infection: GATA motif is recognized by an insect protein. J Virol. 1992 Jun;66(6):3494–3503. doi: 10.1128/jvi.66.6.3494-3503.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Krappa R., Knebel-Mörsdorf D. Identification of the very early transcribed baculovirus gene PE-38. J Virol. 1991 Feb;65(2):805–812. doi: 10.1128/jvi.65.2.805-812.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Lu A., Carstens E. B. Nucleotide sequence and transcriptional analysis of the p80 gene of Autographa californica nuclear polyhedrosis virus: a homologue of the Orgyia pseudotsugata nuclear polyhedrosis virus capsid-associated gene. Virology. 1992 Sep;190(1):201–209. doi: 10.1016/0042-6822(92)91206-a. [DOI] [PubMed] [Google Scholar]
  21. Lu A., Carstens E. B. Transcription analysis of the EcoRI D region of the baculovirus Autographa californica nuclear polyhedrosis virus identifies an early 4-kilobase RNA encoding the essential p143 gene. J Virol. 1992 Feb;66(2):655–663. doi: 10.1128/jvi.66.2.655-663.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Nissen M. S., Friesen P. D. Molecular analysis of the transcriptional regulatory region of an early baculovirus gene. J Virol. 1989 Feb;63(2):493–503. doi: 10.1128/jvi.63.2.493-503.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Pearson M., Bjornson R., Pearson G., Rohrmann G. The Autographa californica baculovirus genome: evidence for multiple replication origins. Science. 1992 Sep 4;257(5075):1382–1384. doi: 10.1126/science.1529337. [DOI] [PubMed] [Google Scholar]
  24. Pearson W. R., Lipman D. J. Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A. 1988 Apr;85(8):2444–2448. doi: 10.1073/pnas.85.8.2444. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Rice W. C., Miller L. K. Baculovirus transcription in the presence of inhibitors and in nonpermissive Drosophila cells. Virus Res. 1986 Nov;6(2):155–172. doi: 10.1016/0168-1702(86)90047-x. [DOI] [PubMed] [Google Scholar]
  26. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Thiem S. M., Miller L. K. A baculovirus gene with a novel transcription pattern encodes a polypeptide with a zinc finger and a leucine zipper. J Virol. 1989 Nov;63(11):4489–4497. doi: 10.1128/jvi.63.11.4489-4497.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Tjia S. T., Carstens E. B., Doerfler W. Infection of Spodoptera frugiperda cells with Autographa californica nuclear polyhedrosis virus II. The viral DNA and the kinetics of its replication. Virology. 1979 Dec;99(2):399–409. doi: 10.1016/0042-6822(79)90018-7. [DOI] [PubMed] [Google Scholar]
  29. Vaughn J. L., Goodwin R. H., Tompkins G. J., McCawley P. The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae). In Vitro. 1977 Apr;13(4):213–217. doi: 10.1007/BF02615077. [DOI] [PubMed] [Google Scholar]
  30. Vlak J. M., Smith G. E. Orientation of the Genome of Autographa californica Nuclear Polyhedrosis Virus: a Proposal. J Virol. 1982 Mar;41(3):1118–1121. doi: 10.1128/jvi.41.3.1118-1121.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Yang C. L., Stetler D. A., Weaver R. F. Structural comparison of the Autographa californica nuclear polyhedrosis virus-induced RNA polymerase and the three nuclear RNA polymerases from the host, Spodoptera frugiperda. Virus Res. 1991 Aug;20(3):251–264. doi: 10.1016/0168-1702(91)90079-b. [DOI] [PubMed] [Google Scholar]

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