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. 1993 Oct;67(10):6234–6238. doi: 10.1128/jvi.67.10.6234-6238.1993

Host range expansion of Autographa californica nuclear polyhedrosis virus (NPV) following recombination of a 0.6-kilobase-pair DNA fragment originating from Bombyx mori NPV.

S Maeda 1, S G Kamita 1, A Kondo 1
PMCID: PMC238045  PMID: 8396678

Abstract

We have isolated hybrid baculoviruses of Bombyx mori nuclear polyhedrosis virus (BmNPV) and Autographa californica NPV (AcNPV) capable of replicating in both BmN (not susceptible to AcNPV) and SF-21 (not susceptible to BmNPV) cells (A. Kondo and S. Maeda, J. Virol. 65:3625-3632, 1991). Repeated backcross infection of one of these recombinant isolates with AcNPV generated eh-AcNPV, a virus with restriction endonuclease patterns of genomic DNA nearly identical to those of AcNPV but capable of replicating in both BmN and SF-21 cells, i.e., host range expanded. Expanded host range viruses were also isolated following cotransfection of AcNPV DNA with eh-AcNPV DNA cleaved with either HindIII or PstI. Subsequent cotransfection of AcNPV DNA with plasmids from an eh-AcNPV DNA fragment library identified an 11-kbp HindIII fragment that could expand the host range of AcNPV. Subcloning and cotransfection analyses localized a 572-bp SacI-HindIII fragment within this 11-kbp fragment which could alone expand the host range of AcNPV. Mapping and nucleotide sequencing analysis revealed that this fragment was identical to the corresponding 572-bp fragment (BmScH) of BmNPV. Furthermore, this fragment originated from the coding region of the putative DNA helicase gene. Cotransfection of AcNPV DNA with BmScH also generated a host range-expanded virus, eh2-AcNPV. These results indicated that the expanded host range characteristics of eh2-AcNPV were solely the result of recombination within the coding region of the putative DNA helicase gene.

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Selected References

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  1. Carbonell L. F., Miller L. K. Baculovirus interaction with nontarget organisms: a virus-borne reporter gene is not expressed in two mammalian cell lines. Appl Environ Microbiol. 1987 Jul;53(7):1412–1417. doi: 10.1128/aem.53.7.1412-1417.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Christensen J., Storgaard T., Bloch B., Alexandersen S., Aasted B. Expression of Aleutian mink disease parvovirus proteins in a baculovirus vector system. J Virol. 1993 Jan;67(1):229–238. doi: 10.1128/jvi.67.1.229-238.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Huybrechts R., Guarino L., Van Brussel M., Vulsteke V. Nucleotide sequence of a transactivating Bombyx mori nuclear polyhedrosis virus immediate early gene. Biochim Biophys Acta. 1992 Feb 11;1129(3):328–330. doi: 10.1016/0167-4781(92)90511-w. [DOI] [PubMed] [Google Scholar]
  4. Iatrou K., Ito K., Witkiewicz H. Polyhedrin gene of Bombyx mori nuclear polyhedrosis virus. J Virol. 1985 May;54(2):436–445. doi: 10.1128/jvi.54.2.436-445.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Im D. S., Muzyczka N. The AAV origin binding protein Rep68 is an ATP-dependent site-specific endonuclease with DNA helicase activity. Cell. 1990 May 4;61(3):447–457. doi: 10.1016/0092-8674(90)90526-k. [DOI] [PubMed] [Google Scholar]
  6. Kamita S. G., Maeda S. Inhibition of Bombyx mori nuclear polyhedrosis virus (NPV) replication by the putative DNA helicase gene of Autographa californica NPV. J Virol. 1993 Oct;67(10):6239–6245. doi: 10.1128/jvi.67.10.6239-6245.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kamita S. G., Majima K., Maeda S. Identification and characterization of the p35 gene of Bombyx mori nuclear polyhedrosis virus that prevents virus-induced apoptosis. J Virol. 1993 Jan;67(1):455–463. doi: 10.1128/jvi.67.1.455-463.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kondo A., Maeda S. Host range expansion by recombination of the baculoviruses Bombyx mori nuclear polyhedrosis virus and Autographa californica nuclear polyhedrosis virus. J Virol. 1991 Jul;65(7):3625–3632. doi: 10.1128/jvi.65.7.3625-3632.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Legendre D., Rommelaere J. Terminal regions of the NS-1 protein of the parvovirus minute virus of mice are involved in cytotoxicity and promoter trans inhibition. J Virol. 1992 Oct;66(10):5705–5713. doi: 10.1128/jvi.66.10.5705-5713.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Li X., Rhode S. L., 3rd Mutation of lysine 405 to serine in the parvovirus H-1 NS1 abolishes its functions for viral DNA replication, late promoter trans activation, and cytotoxicity. J Virol. 1990 Oct;64(10):4654–4660. doi: 10.1128/jvi.64.10.4654-4660.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lu A., Carstens E. B. Nucleotide sequence of a gene essential for viral DNA replication in the baculovirus Autographa californica nuclear polyhedrosis virus. Virology. 1991 Mar;181(1):336–347. doi: 10.1016/0042-6822(91)90500-b. [DOI] [PubMed] [Google Scholar]
  12. Maeda S., Kamita S. G., Kataoka H. The basic DNA-binding protein of Bombyx mori nuclear polyhedrosis virus: the existence of an additional arginine repeat. Virology. 1991 Feb;180(2):807–810. doi: 10.1016/0042-6822(91)90096-t. [DOI] [PubMed] [Google Scholar]
  13. Maeda S., Kawai T., Obinata M., Fujiwara H., Horiuchi T., Saeki Y., Sato Y., Furusawa M. Production of human alpha-interferon in silkworm using a baculovirus vector. Nature. 1985 Jun 13;315(6020):592–594. doi: 10.1038/315592a0. [DOI] [PubMed] [Google Scholar]
  14. Maeda S., Majima K. Molecular cloning and physical mapping of the genome of Bombyx mori nuclear polyhedrosis virus. J Gen Virol. 1990 Aug;71(Pt 8):1851–1855. doi: 10.1099/0022-1317-71-8-1851. [DOI] [PubMed] [Google Scholar]
  15. Maeda S., Mukohara Y., Kondo A. Characteristically distinct isolates of the nuclear polyhedrosis virus from Spodoptera litura. J Gen Virol. 1990 Nov;71(Pt 11):2631–2639. doi: 10.1099/0022-1317-71-11-2631. [DOI] [PubMed] [Google Scholar]
  16. Matson S. W., Kaiser-Rogers K. A. DNA helicases. Annu Rev Biochem. 1990;59:289–329. doi: 10.1146/annurev.bi.59.070190.001445. [DOI] [PubMed] [Google Scholar]
  17. Vasudevacharya J., Compans R. W. The NS and capsid genes determine the host range of porcine parvovirus. Virology. 1992 Apr;187(2):515–524. doi: 10.1016/0042-6822(92)90454-w. [DOI] [PubMed] [Google Scholar]
  18. Vaughn J. L., Goodwin R. H., Tompkins G. J., McCawley P. The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae). In Vitro. 1977 Apr;13(4):213–217. doi: 10.1007/BF02615077. [DOI] [PubMed] [Google Scholar]
  19. Whitford M., Faulkner P. Nucleotide sequence and transcriptional analysis of a gene encoding gp41, a structural glycoprotein of the baculovirus Autographa californica nuclear polyhedrosis virus. J Virol. 1992 Aug;66(8):4763–4768. doi: 10.1128/jvi.66.8.4763-4768.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wilson G. M., Jindal H. K., Yeung D. E., Chen W., Astell C. R. Expression of minute virus of mice major nonstructural protein in insect cells: purification and identification of ATPase and helicase activities. Virology. 1991 Nov;185(1):90–98. doi: 10.1016/0042-6822(91)90757-3. [DOI] [PubMed] [Google Scholar]

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