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. 1993 Nov;67(11):6642–6647. doi: 10.1128/jvi.67.11.6642-6647.1993

A conserved neutralizing epitope on gp41 of human immunodeficiency virus type 1.

T Muster 1, F Steindl 1, M Purtscher 1, A Trkola 1, A Klima 1, G Himmler 1, F Rüker 1, H Katinger 1
PMCID: PMC238102  PMID: 7692082

Abstract

Vaccination against human immunodeficiency virus type 1 (HIV-1) requires an immunogen which will elicit a protective immunity against viruses that show a high degree of genetic polymorphism. Therefore, the identification of neutralizing epitopes which are shared by many strains would be useful. In previous studies, we established a human monoclonal antibody (2F5) that neutralizes a variety of laboratory strains and clinical isolates of HIV-1. In the present report, we define the amino acid sequence Glu-Leu-Asp-Lys-Trp-Ala (ELDKWA) on the ectodomain of gp41 as the epitope recognized by this antibody. The sequence was found to be conserved in 72% of otherwise highly variable HIV-1 isolates. Escape mutants were not detected in cells infected with HIV-1 isolates MN and RF in the presence of antibody 2F5. Since sequence variability of neutralizing epitopes is considered to be a major obstacle to HIV-1 vaccine development, the conserved B-cell epitope described here is a promising candidate for inclusion in a vaccine against AIDS.

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Selected References

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