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. 1985 Jan;49(1):109–114. doi: 10.1128/aem.49.1.109-114.1985

Role of Chemotaxis in the Ecology of Denitrifiers

Michael J Kennedy 1,†,*, James G Lawless 1
PMCID: PMC238353  PMID: 16346690

Abstract

A modification of the Adler capillary assay was used to evaluate the chemotactic responses of several denitrifiers to nitrate and nitrite. Strong positive chemotaxis was observed to NO3− and NO2− by soil isolates of Pseudomonas aeruginosa, Pseudomonas fluorescens, and Pseudomonas stutzeri, with the peak response occurring at 10−3 M for both attractants. In addition, a strong chemoattraction to serine (peak response at 10−2 M), tryptone, and a soil extract, but not to NH4+, was observed for all denitrifiers tested. Chemotaxis was not dependent on a previous growth on NO3−, NO2−, or a soil extract, and the chemoattraction to NO3− occurred when the bacteria were grown aerobically or anaerobically. However, the best response to NO3− was usually observed when the cells were grown aerobically with 10 mM NO3− in the growth medium. Capillary tubes containing 10−3 M NO3− submerged into soil-water mixtures elicited a significant chemotactic response to NO3− by the indigenous soil microflora, the majority of which were Pseudomonas spp. A chemotactic strain of P. fluorescens also was shown to survive significantly better in aerobic and anaerobic soils than was a nonmotile strain of the same species. Both strains had equal growth rates in liquid cultures. Thus, chemotaxis may be one mechanism by which denitrifiers successfully compete for available NO3− and NO2−, and which may facilitate the survival of naturally occurring populations of some denitrifiers.

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Selected References

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  1. Adler J. A method for measuring chemotaxis and use of the method to determine optimum conditions for chemotaxis by Escherichia coli. J Gen Microbiol. 1973 Jan;74(1):77–91. doi: 10.1099/00221287-74-1-77. [DOI] [PubMed] [Google Scholar]
  2. Adler J. Chemotaxis in bacteria. Annu Rev Biochem. 1975;44:341–356. doi: 10.1146/annurev.bi.44.070175.002013. [DOI] [PubMed] [Google Scholar]
  3. Betlach M. R., Tiedje J. M. Kinetic explanation for accumulation of nitrite, nitric oxide, and nitrous oxide during bacterial denitrification. Appl Environ Microbiol. 1981 Dec;42(6):1074–1084. doi: 10.1128/aem.42.6.1074-1084.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chet I., Mitchell R. Ecological aspects of microbial chemotactic behavior. Annu Rev Microbiol. 1976;30:221–239. doi: 10.1146/annurev.mi.30.100176.001253. [DOI] [PubMed] [Google Scholar]
  5. Currier W. W., Strobel G. A. Chemotaxis of Rhizobium spp. to Plant Root Exudates. Plant Physiol. 1976 May;57(5):820–823. doi: 10.1104/pp.57.5.820. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Freter R., O'Brien P. C., Halstead S. A. Adhesion and chemotaxis as determinants of bacterial association with mucosal surfaces. Adv Exp Med Biol. 1978;107:429–437. doi: 10.1007/978-1-4684-3369-2_48. [DOI] [PubMed] [Google Scholar]
  7. Freter R., O'Brien P. C., Macsai M. S. Role of chemotaxis in the association of motile bacteria with intestinal mucosa: in vivo studies. Infect Immun. 1981 Oct;34(1):234–240. doi: 10.1128/iai.34.1.234-240.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Freter R., O'Brien P. C. Role of chemotaxis in the association of motile bacteria with intestinal mucosa: chemotactic responses of Vibrio cholerae and description of motile nonchemotactic mutants. Infect Immun. 1981 Oct;34(1):215–221. doi: 10.1128/iai.34.1.215-221.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gallucci K. K., Paerl H. W. Pseudomonas aeruginosa Chemotaxis Associated with Blooms of N(2)-Fixing Blue-Green Algae (Cyanobacteria). Appl Environ Microbiol. 1983 Feb;45(2):557–562. doi: 10.1128/aem.45.2.557-562.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gamble T. N., Betlach M. R., Tiedje J. M. Numerically dominant denitrifying bacteria from world soils. Appl Environ Microbiol. 1977 Apr;33(4):926–939. doi: 10.1128/aem.33.4.926-939.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Grant M. A., Holt J. G. Medium for the selective isolation of members of the genus Pseudomonas from natural habitats. Appl Environ Microbiol. 1977 May;33(5):1222–1224. doi: 10.1128/aem.33.5.1222-1224.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lauffenburger D., Aris R., Keller K. Effects of cell motility and chemotaxis on microbial population growth. Biophys J. 1982 Dec;40(3):209–219. doi: 10.1016/S0006-3495(82)84476-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lynch W. H. Effect of temperature on Pseudomonas fluorescens chemotaxis. J Bacteriol. 1980 Jul;143(1):338–342. doi: 10.1128/jb.143.1.338-342.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Moulton R. C., Montie T. C. Chemotaxis by Pseudomonas aeruginosa. J Bacteriol. 1979 Jan;137(1):274–280. doi: 10.1128/jb.137.1.274-280.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Payne W. J. Reduction of nitrogenous oxides by microorganisms. Bacteriol Rev. 1973 Dec;37(4):409–452. doi: 10.1128/br.37.4.409-452.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Pilgram W. K., Williams F. D. Survival value of chemotaxis in mixed cultures. Can J Microbiol. 1976 Dec;22(12):1771–1773. doi: 10.1139/m76-262. [DOI] [PubMed] [Google Scholar]
  17. Sjoblad R. D., Mitchell R. Chemotactic responses of Vibrio alginolyticus to algal extracellular products. Can J Microbiol. 1979 Sep;25(9):964–967. doi: 10.1139/m79-147. [DOI] [PubMed] [Google Scholar]
  18. Soby S., Bergman K. Motility and Chemotaxis of Rhizobium meliloti in Soil. Appl Environ Microbiol. 1983 Nov;46(5):995–998. doi: 10.1128/aem.46.5.995-998.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Tiedje J. M., Sexstone A. J., Myrold D. D., Robinson J. A. Denitrification: ecological niches, competition and survival. Antonie Van Leeuwenhoek. 1982;48(6):569–583. doi: 10.1007/BF00399542. [DOI] [PubMed] [Google Scholar]

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