Abstract
N-methyl-N'-nitro-N-nitrosoguanidine (nitrosoguanidine) and to a lesser extent UV radiation are very mutagenic for Gibberella microconidia. The recommended nitrosoguanidine doses lead to much higher frequencies of mutants than are found in other microorganisms. The frequency of mutants among the survivors increases linearly with the nitrosoguanidine dose (molar concentration X time); the absolute number of viable mutants in a given population reaches a maximum for a dose of ca. 0.7 M X s. The microconidia are uninucleate. The onset of germination brings about increased lethality of nitrosoguanidine, but it does not modify the action of UV radiation. Mycelia are more resistant than spores to both agents. Visible illumination effectively prevents lethality when given immediately after UV radiation. Auxotrophs and color mutants are very easily obtained. Pink adenine auxotrophs and several classes of color mutants are affected in the biosynthesis of the carotenoid pigment, neurosporaxanthin.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Calderón I. L., Cerdá-Olmedo E. Induction by N-methyl-N'-nitro-N-nitrosoguanidine of nuclear and cytoplasmic mutations in Saccharomyces cerevisiae. Mutat Res. 1983 Mar;108(1-3):133–146. doi: 10.1016/0027-5107(83)90115-x. [DOI] [PubMed] [Google Scholar]
- Cerdá-Olmedo E., Hanawalt P. C., Guerola N. Mutagenesis of the replication point by nitrosoguanidine: map and pattern of replication of the Escherichia coli chromosome. J Mol Biol. 1968 May 14;33(3):705–719. doi: 10.1016/0022-2836(68)90315-x. [DOI] [PubMed] [Google Scholar]
- Cerdá-Olmedo E., Hanawalt P. C. The replication of the Escherichia coli chromosome studied by sequential nitrosoguanidine mutagenesis. Cold Spring Harb Symp Quant Biol. 1968;33:599–607. doi: 10.1101/sqb.1968.033.01.066. [DOI] [PubMed] [Google Scholar]
- Cerdá-Olmedo E., Reau P. Genetic classification of the lethal effects of various agents on heterokaryotic spores of Phycomyces. Mutat Res. 1970 Apr;9(4):369–384. doi: 10.1016/0027-5107(70)90019-9. [DOI] [PubMed] [Google Scholar]
- Dawes I. W., Carter B. L. Nitrosoguanidine mutagenesis during nuclear and mitochondrial gene replication. Nature. 1974 Aug 30;250(5469):709–712. doi: 10.1038/250709a0. [DOI] [PubMed] [Google Scholar]
- Gichner T., Velemínský J. Genetic effects of N-methyl-N'-nitro-N-nitrosoguanidine and its homologs. Mutat Res. 1982 Sep;99(2):129–242. doi: 10.1016/0165-1110(82)90057-4. [DOI] [PubMed] [Google Scholar]
- Parry J. M., Cox B. S. The effects of dark holding and photoreactivation on ultraviolet light-induced mitotic recombination and survival in yeast. Genet Res. 1968 Oct;12(2):187–198. doi: 10.1017/s0016672300011794. [DOI] [PubMed] [Google Scholar]
- Poulter R. T., Rikkerink E. H. Genetic analysis of red, adenine-requiring mutants of Candida albicans. J Bacteriol. 1983 Dec;156(3):1066–1077. doi: 10.1128/jb.156.3.1066-1077.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roncero M. I., Zabala C., Cerdá-Olmedo E. Mutagenesis in multinucleate cells: the effects of N-methyl-N'-nitro-N-nitrosoguanidine on Phycomyces spores. Mutat Res. 1984 Feb;125(2):195–204. doi: 10.1016/0027-5107(84)90069-1. [DOI] [PubMed] [Google Scholar]
- Silver J. M., Eaton N. R. Functional blocks of the ad-1 and ad-2 mutants of Saccharomyces cerevisiae. Biochem Biophys Res Commun. 1969 Feb 7;34(3):301–305. doi: 10.1016/0006-291x(69)90831-6. [DOI] [PubMed] [Google Scholar]
- Spector C., Phinney B. O. Gibberellin Production: Genetic Control in the Fungus Gibberelia fujikuroi. Science. 1966 Sep 16;153(3742):1397–1398. doi: 10.1126/science.153.3742.1397. [DOI] [PubMed] [Google Scholar]