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. 1985 Jan;49(1):192–197. doi: 10.1128/aem.49.1.192-197.1985

Tolerance of Acinetobacter calcoaceticus RAG-1 to the cationic surfactant cetyltrimethylammonium bromide: role of the bioemulsifier emulsan.

Y Shabtai, D L Gutnick
PMCID: PMC238368  PMID: 3838426

Abstract

Emulsan, the polyanionic heteropolysaccharide bioemulsifier produced by Acinetobacter calcoaceticus RAG-1, was found to enhance the tolerance of RAG-1 cells to the toxic effects of the cationic detergent cetyltrimethylammonium bromide (CTAB). Emulsan-mediated tolerance was obtained with the purified deproteinated apoemulsan; ca. 9 micrograms of apoemulsan neutralized 1 microgram of CTAB. Deesterified apoemulsan was only half as effective in protecting the cells from CTAB toxicity. Tolerance was also mediated by the cell-associated emulsan minicapsule. Mutants lacking this capsule were more sensitive to CTAB than the corresponding parent. The growth of mutants and parent cells in mixed-culture experiments demonstrated that the cell-associated polymer mediates CTAB tolerance in the early stages of growth. Once sufficient cell-free polymer has been released into the aqueous medium (ca. 0.5 micrograms/ml), this extracellular emulsan also plays a role in CTAB tolerance.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bayer E. A., Rosenberg E., Gutnick D. The isolation of cell surface mutants of Acinetobacter calcoaceticus RAG-1. J Gen Microbiol. 1981 Dec;127(2):295–300. doi: 10.1099/00221287-127-2-295. [DOI] [PubMed] [Google Scholar]
  2. Belsky I., Gutnick D. L., Rosenberg E. Emulsifier of Arthrobacter RAG-1: determination of emulsifier-bound fatty acids. FEBS Lett. 1979 May 1;101(1):175–178. doi: 10.1016/0014-5793(79)81320-4. [DOI] [PubMed] [Google Scholar]
  3. Costerton J. W., Irvin R. T., Cheng K. J. The role of bacterial surface structures in pathogenesis. Crit Rev Microbiol. 1981;8(4):303–338. doi: 10.3109/10408418109085082. [DOI] [PubMed] [Google Scholar]
  4. Goldman S., Shabtai Y., Rubinovitz C., Rosenberg E., Gutnick D. L. Emulsan in Acinetobacter calcoaceticus RAG-1: Distribution of Cell-Free and Cell-Associated Cross-Reacting Material. Appl Environ Microbiol. 1982 Jul;44(1):165–170. doi: 10.1128/aem.44.1.165-170.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Govan J. R., Fyfe J. A. Mucoid Pseudomonas aeruginosa and cystic fibrosis: resistance of the mucoid from to carbenicillin, flucloxacillin and tobramycin and the isolation of mucoid variants in vitro. J Antimicrob Chemother. 1978 May;4(3):233–240. doi: 10.1093/jac/4.3.233. [DOI] [PubMed] [Google Scholar]
  6. Govan J. R. Mucoid strains of Pseudomonas aeruginosa: the influence of culture medium on the stability of mucus production. J Med Microbiol. 1975 Nov;8(4):513–522. doi: 10.1099/00222615-8-4-513. [DOI] [PubMed] [Google Scholar]
  7. Gutnick D. L., Rosenberg E. Oil tankers and pollution: a microbiological approach. Annu Rev Microbiol. 1977;31:379–396. doi: 10.1146/annurev.mi.31.100177.002115. [DOI] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Pines O., Bayer E. A., Gutnick D. L. Localization of emulsan-like polymers associated with the cell surface of acinetobacter calcoaceticus. J Bacteriol. 1983 May;154(2):893–905. doi: 10.1128/jb.154.2.893-905.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Pines O., Gutnick D. Specific binding of a bacteriophage at a hydrocarbon-water interface. J Bacteriol. 1984 Jan;157(1):179–183. doi: 10.1128/jb.157.1.179-183.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Reisfeld A., Rosenberg E., Gutnick D. Microbial degradation of crude oil: factors affecting the dispersion in sea water by mixed and pure cultures. Appl Microbiol. 1972 Sep;24(3):363–368. doi: 10.1128/am.24.3.363-368.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rosenberg E., Perry A., Gibson D. T., Gutnick D. L. Emulsifier of Arthrobacter RAG-1: specificity of hydrocarbon substrate. Appl Environ Microbiol. 1979 Mar;37(3):409–413. doi: 10.1128/aem.37.3.409-413.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rosenberg E., Zuckerberg A., Rubinovitz C., Gutnick D. L. Emulsifier of Arthrobacter RAG-1: isolation and emulsifying properties. Appl Environ Microbiol. 1979 Mar;37(3):402–408. doi: 10.1128/aem.37.3.402-408.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rubinovitz C., Gutnick D. L., Rosenberg E. Emulsan production by Acinetobacter calcoaceticus in the presence of chloramphenicol. J Bacteriol. 1982 Oct;152(1):126–132. doi: 10.1128/jb.152.1.126-132.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. SALTON M. R. J., HORNE R. W., COSSLETT V. E. Electron microscopy of bacteria treated with cetyltrimethylammonium bromide. J Gen Microbiol. 1951 May;5(2):405–407. doi: 10.1099/00221287-5-2-405. [DOI] [PubMed] [Google Scholar]
  16. SALTON M. R. J. The adsorption of cetyltrimethylammonium bromide by bacteria, its action in releasing cellular constituents and its bactericidal effects. J Gen Microbiol. 1951 May;5(2):391–404. doi: 10.1099/00221287-5-2-391. [DOI] [PubMed] [Google Scholar]
  17. Ulitzur S., Shilo M. Effect of Prymnesium parvum toxin, cetyltrimethylammonium bromide and sodium dodecyl sulphate on bacteria. J Gen Microbiol. 1970 Aug;62(3):363–370. doi: 10.1099/00221287-62-3-363. [DOI] [PubMed] [Google Scholar]
  18. Zuckerberg A., Diver A., Peeri Z., Gutnick D. L., Rosenberg E. Emulsifier of Arthrobacter RAG-1: chemical and physical properties. Appl Environ Microbiol. 1979 Mar;37(3):414–420. doi: 10.1128/aem.37.3.414-420.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

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