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. 1985 Apr;49(4):1001–1003. doi: 10.1128/aem.49.4.1001-1003.1985

General Method for the Identification of Plasmid Species in Fast-Growing Soil Microorganisms

Jacek Plazinski 1,*, Ying H Cen 1, Barry G Rolfe 1
PMCID: PMC238487  PMID: 16346763

Abstract

Using a horizontal gel electrophoresis method, we demonstrated reproducibly the presence of indigenous plasmids in different Rhizobium, Agrobacterium, and Pseudomonas strains. The method yields a large amount of plasmid DNA and is sensitive in detecting megaplasmids with molecular weights higher than 5 × 108. In two Rhizobium meliloti strains, a megaplasmid other than the low-mobility plasmid already known was detected.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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