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. 1985 Jul;50(1):21–26. doi: 10.1128/aem.50.1.21-26.1985

Enhanced chlorine resistance of tap water-adapted Legionella pneumophila as compared with agar medium-passaged strains.

J M Kuchta, S J States, J E McGlaughlin, J H Overmeyer, R M Wadowsky, A M McNamara, R S Wolford, R B Yee
PMCID: PMC238566  PMID: 3896142

Abstract

Previous studies have shown that bacteria maintained in a low-nutrient "natural" environment such as swimming pool water are much more resistant to disinfection by various chemical agents than strains maintained on rich media. In the present study a comparison was made of the chlorine (Cl2) susceptibility of hot-water tank isolates of Legionella pneumophila maintained in tap water and strains passaged on either nonselective buffered charcoal-yeast extract or selective differential glycine-vancomycin-polymyxin agar medium. Our earlier work has shown that environmental and clinical isolates of L. pneumophila maintained on agar medium are much more resistant to Cl2 than coliforms are. Under the present experimental conditions (21 degrees C, pH 7.6 to 8.0, and 0.25 mg of free residual Cl2 per liter, we found the tap water-maintained L. pneumophila strains to be even more resistant than the agar-passaged isolates. Under these conditions, 99% kill of tap water-maintained strains of L. pneumophila was usually achieved within 60 to 90 min compared with 10 min for agar-passaged strains. Samples from plumbing fixtures in a hospital yielded legionellae which were "super"-chlorine resistant when assayed under natural conditions. After one agar passage their resistance dropped to levels of comparable strains which had not been previously exposed to additional chlorination. These studies more closely approximate natural conditions than our previous work and show that tap water-maintained L. pneumophila is even more resistant to Cl2 than its already resistant agar medium-passaged counterpart.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aieta E. M., Berg J. D., Roberts P. V., Cooper R. C. Comparison of chlorine dioxide and chlorine in wastewater disinfection. J Water Pollut Control Fed. 1980 Apr;52(4):810–824. [PubMed] [Google Scholar]
  2. Berg J. D., Matin A., Roberts P. V. Effect of antecedent growth conditions on sensitivity of Escherichia coli to chlorine dioxide. Appl Environ Microbiol. 1982 Oct;44(4):814–819. doi: 10.1128/aem.44.4.814-819.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bohach G. A., Snyder I. S. Characterization of surfaces involved in adherence of Legionella pneumophila to Fischerella species. Infect Immun. 1983 Oct;42(1):318–325. doi: 10.1128/iai.42.1.318-325.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Carson L. A., Favero M. S., Bond W. W., Petersen N. J. Factors affecting comparative resistance of naturally occurring and subcultured Pseudomonas aeruginosa to disinfectants. Appl Microbiol. 1972 May;23(5):863–869. doi: 10.1128/am.23.5.863-869.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Carson L. A., Petersen N. J., Favero M. S., Aguero S. M. Growth characteristics of atypical mycobacteria in water and their comparative resistance to disinfectants. Appl Environ Microbiol. 1978 Dec;36(6):839–846. doi: 10.1128/aem.36.6.839-846.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chai T. J. Characteristics of Escherichia coli grown in bay water as compared with rich medium. Appl Environ Microbiol. 1983 Apr;45(4):1316–1323. doi: 10.1128/aem.45.4.1316-1323.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cherry W. B., Pittman B., Harris P. P., Hebert G. A., Thomason B. M., Thacker L., Weaver R. E. Detection of Legionnaires disease bacteria by direct immunofluorescent staining. J Clin Microbiol. 1978 Sep;8(3):329–338. doi: 10.1128/jcm.8.3.329-338.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dondero T. J., Jr, Rendtorff R. C., Mallison G. F., Weeks R. M., Levy J. S., Wong E. W., Schaffner W. An outbreak of Legionnaires' disease associated with a contaminated air-conditioning cooling tower. N Engl J Med. 1980 Feb 14;302(7):365–370. doi: 10.1056/NEJM198002143020703. [DOI] [PubMed] [Google Scholar]
  9. Favero M. S., Drake C. H. Factors influencing the occurrence of high numbers of iodine-resistant bacteria in iodinated swimming pools. Appl Microbiol. 1966 Jul;14(4):627–635. doi: 10.1128/am.14.4.627-635.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Finnerty W. R., Makula R. A., Feeley J. C. Cellular lipids of the Legionnaires' disease bacterium. Ann Intern Med. 1979 Apr;90(4):631–634. doi: 10.7326/0003-4819-90-4-631. [DOI] [PubMed] [Google Scholar]
  11. Fliermans C. B., Cherry W. B., Orrison L. H., Thacker L. Isolation of Legionella pneumophila from nonepidemic-related aquatic habitats. Appl Environ Microbiol. 1979 Jun;37(6):1239–1242. doi: 10.1128/aem.37.6.1239-1242.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Goldfine H. Comparative aspects of bacterial lipids. Adv Microb Physiol. 1972;8:1–58. doi: 10.1016/s0065-2911(08)60187-3. [DOI] [PubMed] [Google Scholar]
  13. Hindahl M. S., Iglewski B. H. Isolation and characterization of the Legionella pneumophila outer membrane. J Bacteriol. 1984 Jul;159(1):107–113. doi: 10.1128/jb.159.1.107-113.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kuchta J. M., States S. J., McNamara A. M., Wadowsky R. M., Yee R. B. Susceptibility of Legionella pneumophila to chlorine in tap water. Appl Environ Microbiol. 1983 Nov;46(5):1134–1139. doi: 10.1128/aem.46.5.1134-1139.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. MILBAUER R., GROSSOWICZ N. Effect of growth conditions on chlorine sensitivity of Escherichia coli. Appl Microbiol. 1959 Mar;7(2):71–74. doi: 10.1128/am.7.2.71-74.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Morris G. K., Patton C. M., Feeley J. C., Johnson S. E., Gorman G., Martin W. T., Skaliy P., Mallison G. F., Politi B. D., Mackel D. C. Isolation of the Legionnaires' disease bacterium from environmental samples. Ann Intern Med. 1979 Apr;90(4):664–666. doi: 10.7326/0003-4819-90-4-664. [DOI] [PubMed] [Google Scholar]
  17. Pasculle A. W., Feeley J. C., Gibson R. J., Cordes L. G., Myerowitz R. L., Patton C. M., Gorman G. W., Carmack C. L., Ezzell J. W., Dowling J. N. Pittsburgh pneumonia agent: direct isolation from human lung tissue. J Infect Dis. 1980 Jun;141(6):727–732. doi: 10.1093/infdis/141.6.727. [DOI] [PubMed] [Google Scholar]
  18. Pohlod D. J., Saravolatz L. D., Quinn E. L., Somerville M. M. The effect of inoculum, culture medium and antimicrobial combinations on the in-vitro susceptibilities of Legionella pneumophila. J Antimicrob Chemother. 1981 Apr;7(4):335–341. doi: 10.1093/jac/7.4.335. [DOI] [PubMed] [Google Scholar]
  19. Politi B. D., Fraser D. W., Mallison G. F., Mohatt J. V., Morris G. K., Patton C. M., Feeley J. C., Telle R. D., Bennett J. V. A major focus of Legionnaires' disease in Bloomington, Indiana. Ann Intern Med. 1979 Apr;90(4):587–591. doi: 10.7326/0003-4819-90-4-587. [DOI] [PubMed] [Google Scholar]
  20. Ridgway H. F., Olson B. H. Chlorine resistance patterns of bacteria from two drinking water distribution systems. Appl Environ Microbiol. 1982 Oct;44(4):972–987. doi: 10.1128/aem.44.4.972-987.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wadowsky R. M., Yee R. B. Glycine-containing selective medium for isolation of Legionellaceae from environmental specimens. Appl Environ Microbiol. 1981 Nov;42(5):768–772. doi: 10.1128/aem.42.5.768-772.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wadowsky R. M., Yee R. B., Mezmar L., Wing E. J., Dowling J. N. Hot water systems as sources of Legionella pneumophila in hospital and nonhospital plumbing fixtures. Appl Environ Microbiol. 1982 May;43(5):1104–1110. doi: 10.1128/aem.43.5.1104-1110.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wadowsky R. M., Yee R. B. Satellite growth of Legionella pneumophila with an environmental isolate of Flavobacterium breve. Appl Environ Microbiol. 1983 Dec;46(6):1447–1449. doi: 10.1128/aem.46.6.1447-1449.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Yee R. B., Wadowsky R. M. Multiplication of Legionella pneumophila in unsterilized tap water. Appl Environ Microbiol. 1982 Jun;43(6):1330–1334. doi: 10.1128/aem.43.6.1330-1334.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]

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