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. 1985 Nov;50(5):1244–1250. doi: 10.1128/aem.50.5.1244-1250.1985

Interactions in Syntrophic Associations of Endospore-Forming, Butyrate-Degrading Bacteria and H2-Consuming Bacteria

Francisco A Tomei 1,†, James S Maki 1, Ralph Mitchell 1,*
PMCID: PMC238733  PMID: 16346930

Abstract

Butyrate is an important intermediate in the anaerobic degradation of organic matter. In sulfate-depleted environments butyrate is oxidized to acetate and hydrogen by obligate proton reducers, in syntrophic association with hydrogen-consuming methanogens. This paper describes two enrichments of endospore-forming bacteria degrading butyrate in consortia with methanogens. The isolates are readily established in coculture with H2-consuming, sulfate-reducing bacteria by pasteurizing the culture. The two original enrichments differed in that one grew to an optically dense culture while the second grew in clumps. Examination by scanning electron microscopy showed that clumping resulted from the production of large amounts of extracellular polymer. Several H2-consuming methanogens were identified in the enrichments. Some of them grew closely associated to the butyrate degraders. This attachment to the hydrogen producer may permit some methanogens to compete for the growth substrate against other bacteria having higher substrate affinity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Badziong W., Thauer R. K., Zeikus J. G. Isolation and characterization of Desulfovibrio growing on hydrogen plus sulfate as the sole energy source. Arch Microbiol. 1978 Jan 23;116(1):41–49. doi: 10.1007/BF00408732. [DOI] [PubMed] [Google Scholar]
  2. Balch W. E., Fox G. E., Magrum L. J., Woese C. R., Wolfe R. S. Methanogens: reevaluation of a unique biological group. Microbiol Rev. 1979 Jun;43(2):260–296. doi: 10.1128/mr.43.2.260-296.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Balch W. E., Wolfe R. S. New approach to the cultivation of methanogenic bacteria: 2-mercaptoethanesulfonic acid (HS-CoM)-dependent growth of Methanobacterium ruminantium in a pressureized atmosphere. Appl Environ Microbiol. 1976 Dec;32(6):781–791. doi: 10.1128/aem.32.6.781-791.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Balch W. E., Wolfe R. S. Transport of coenzyme M (2-mercaptoethanesulfonic acid) in Methanobacterium ruminantium. J Bacteriol. 1979 Jan;137(1):264–273. doi: 10.1128/jb.137.1.264-273.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Baresi L., Wolfe R. S. Levels of coenzyme F420, coenzyme M, hydrogenase, and methylcoenzyme M methylreductase in acetate-grown Methanosarcina. Appl Environ Microbiol. 1981 Feb;41(2):388–391. doi: 10.1128/aem.41.2.388-391.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Boone D. R. Mixed-culture fermentor for simulating methanogenic digestors. Appl Environ Microbiol. 1984 Jul;48(1):122–126. doi: 10.1128/aem.48.1.122-126.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Bryant M. P., Wolin E. A., Wolin M. J., Wolfe R. S. Methanobacillus omelianskii, a symbiotic association of two species of bacteria. Arch Mikrobiol. 1967;59(1):20–31. doi: 10.1007/BF00406313. [DOI] [PubMed] [Google Scholar]
  8. Ferry J. G., Wolfe R. S. Anaerobic degradation of benzoate to methane by a microbial consortium. Arch Microbiol. 1976 Feb;107(1):33–40. doi: 10.1007/BF00427864. [DOI] [PubMed] [Google Scholar]
  9. Kaspar H. F., Wuhrmann K. Kinetic parameters and relative turnovers of some important catabolic reactions in digesting sludge. Appl Environ Microbiol. 1978 Jul;36(1):1–7. doi: 10.1128/aem.36.1.1-7.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Koch M., Dolfing J., Wuhrmann K., Zehnder A. J. Pathways of propionate degradation by enriched methanogenic cultures. Appl Environ Microbiol. 1983 Apr;45(4):1411–1414. doi: 10.1128/aem.45.4.1411-1414.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lovley D. R., Greening R. C., Ferry J. G. Rapidly growing rumen methanogenic organism that synthesizes coenzyme M and has a high affinity for formate. Appl Environ Microbiol. 1984 Jul;48(1):81–87. doi: 10.1128/aem.48.1.81-87.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mackie R. I., Bryant M. P. Metabolic Activity of Fatty Acid-Oxidizing Bacteria and the Contribution of Acetate, Propionate, Butyrate, and CO(2) to Methanogenesis in Cattle Waste at 40 and 60 degrees C. Appl Environ Microbiol. 1981 Jun;41(6):1363–1373. doi: 10.1128/aem.41.6.1363-1373.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McInerney M. J., Bryant M. P., Hespell R. B., Costerton J. W. Syntrophomonas wolfei gen. nov. sp. nov., an Anaerobic, Syntrophic, Fatty Acid-Oxidizing Bacterium. Appl Environ Microbiol. 1981 Apr;41(4):1029–1039. doi: 10.1128/aem.41.4.1029-1039.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. McInerney M. J., Mackie R. I., Bryant M. P. Syntrophic association of a butyrate-degrading bacterium and methanosarcina enriched from bovine rumen fluid. Appl Environ Microbiol. 1981 Mar;41(3):826–828. doi: 10.1128/aem.41.3.826-828.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Miller T. L., Wolin M. J. A serum bottle modification of the Hungate technique for cultivating obligate anaerobes. Appl Microbiol. 1974 May;27(5):985–987. doi: 10.1128/am.27.5.985-987.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Miller T. L., Wolin M. J., Conway de Macario E., Macario A. J. Isolation of Methanobrevibacter smithii from human feces. Appl Environ Microbiol. 1982 Jan;43(1):227–232. doi: 10.1128/aem.43.1.227-232.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Miller T. L., Wolin M. J. Methanosphaera stadtmaniae gen. nov., sp. nov.: a species that forms methane by reducing methanol with hydrogen. Arch Microbiol. 1985 Mar;141(2):116–122. doi: 10.1007/BF00423270. [DOI] [PubMed] [Google Scholar]
  18. Miller T. L., Wolin M. J. Oxidation of hydrogen and reduction of methanol to methane is the sole energy source for a methanogen isolated from human feces. J Bacteriol. 1983 Feb;153(2):1051–1055. doi: 10.1128/jb.153.2.1051-1055.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Miller T. L., Wolin M. J. Stability of Methanobrevibacter smithii populations in the microbial flora excreted from the human large bowel. Appl Environ Microbiol. 1983 Jan;45(1):317–318. doi: 10.1128/aem.45.1.317-318.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Neill A. R., Grime D. W., Dawson R. M. Conversion of choline methyl groups through trimethylamine into methane in the rumen. Biochem J. 1978 Mar 15;170(3):529–535. doi: 10.1042/bj1700529. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Robinson R. W., Aldrich H. C., Hurst S. F., Bleiweis A. S. Role of the Cell Surface of Methanosarcina mazei in Cell Aggregation. Appl Environ Microbiol. 1985 Feb;49(2):321–327. doi: 10.1128/aem.49.2.321-327.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Shelton D. R., Tiedje J. M. Isolation and partial characterization of bacteria in an anaerobic consortium that mineralizes 3-chlorobenzoic Acid. Appl Environ Microbiol. 1984 Oct;48(4):840–848. doi: 10.1128/aem.48.4.840-848.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Spurr A. R. A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res. 1969 Jan;26(1):31–43. doi: 10.1016/s0022-5320(69)90033-1. [DOI] [PubMed] [Google Scholar]
  25. Widdel F., Pfennig N. Sporulation and further nutritional characteristics of Desulfotomaculum acetoxidans. Arch Microbiol. 1981 Jul;129(5):401–402. doi: 10.1007/BF00406471. [DOI] [PubMed] [Google Scholar]
  26. Zehnder A. J., Huser B. A., Brock T. D., Wuhrmann K. Characterization of an acetate-decarboxylating, non-hydrogen-oxidizing methane bacterium. Arch Microbiol. 1980 Jan;124(1):1–11. doi: 10.1007/BF00407022. [DOI] [PubMed] [Google Scholar]
  27. Zeikus J. G., Bowen V. G. Comparative ultrastructure of methanogenic bacteria. Can J Microbiol. 1975 Feb;21(2):121–129. doi: 10.1139/m75-019. [DOI] [PubMed] [Google Scholar]
  28. Zinder S. H., Cardwell S. C., Anguish T., Lee M., Koch M. Methanogenesis in a Thermophilic (58 degrees C) Anaerobic Digestor: Methanothrix sp. as an Important Aceticlastic Methanogen. Appl Environ Microbiol. 1984 Apr;47(4):796–807. doi: 10.1128/aem.47.4.796-807.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

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