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. 1985 Nov;50(5):1285–1291. doi: 10.1128/aem.50.5.1285-1291.1985

Effect of Fall Turnover on Terminal Carbon Metabolism in Lake Mendota Sediments

T J Phelps 2, J G Zeikus 2,*
PMCID: PMC238740  PMID: 16346933

Abstract

The carbon and electron flow pathways and the bacterial populations responsible for the transformation of H2-CO2, formate, methanol, methylamine, acetate, ethanol, and lactate were examined in eutrophic sediments collected during summer stratification and fall turnover. The rate of methane formation averaged 1,130 μmol of CH4 per liter of sediment per day during late-summer stratification versus 433 μmol of CH4 per liter of sediment per day during the early portion of fall turnover, whereas the rate of sulfate reduction was 280 μmol of sulfate per liter of sediment per day versus 1,840 μmol of sulfate per liter of sediment per day during the same time periods, respectively. The sulfate-reducing population remained constant while the methanogenic population decreased by one to two orders of magnitude during turnover. The acetate concentration increased from 32 to 81 μmol per liter of sediment while the acetate transformation rate constant decreased from 3.22 to 0.70 per h, respectively, during stratification versus turnover. Acetate accounted for nearly 100% of total sedimentary methanogenesis during turnover versus 70% during stratification. The fraction of 14CO2 produced from all 14C-labeled substrates examined was 10 to 40% higher during fall turnover than during stratification. The addition of sulfate, thiosulfate, or sulfur to stratified sediments mimicked fall turnover in that more CO2 and CH4 were produced. The addition of Desulfovibrio vulgaris to sulfate-amended sediments greatly enhanced the amount of CO2 produced from either [14C]methanol or [2-14C]acetate, suggesting that H2 consumption by sulfate reducers can alter methanol or acetate transformation by sedimentary methanogens. These data imply that turnover dynamically altered carbon transformation in eutrophic sediments such that sulfate reduction dominated over methanogenesis principally as a consequence of altering hydrogen metabolism.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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