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. 1985 Dec;50(6):1388–1394. doi: 10.1128/aem.50.6.1388-1394.1985

Characterization and distribution of Vibrio alginolyticus and Vibrio parahaemolyticus isolated in Indonesia.

E Molitoris, S W Joseph, M I Krichevsky, W Sindhuhardja, R R Colwell
PMCID: PMC238769  PMID: 4091566

Abstract

Previous studies have shown that Vibrio alginolyticus and Vibrio parahaemolyticus can be isolated from similar types of marine samples. In this report, the results of an examination of 567 V. alginolyticus and V. parahaemolyticus strains, isolated from seawater in Jakarta Bay and from more than 30 types of seafood from markets in Jakarta, Indonesia, are presented. Most isolates were from mackerel, shrimp, or squid. Numerical taxonomic analyses clustered 337 isolates and three V. alginolyticus reference strains at S greater than or equal to 80%. These strains produced acid from sucrose, but only approximately 80% produced acetoin or grew in the presence of 10% NaCl. The frequency of occurrence of V. alginolyticus in seawater samples ranged from 0% (in February and March 1972) to 100% (in September and December 1972) and was highest in seafood samples from August to December 1972. A second cluster of 230 isolates and seven V. parahaemolyticus reference strains was observed at S greater than or equal to 82%. These strains did not produce acetoin or acid from sucrose, and approximately 20% grew in the presence of 10% NaCl. V. parahaemolyticus was detected in seawater samples each month, with the highest frequency of occurrence (83.3%) in May 1972. Twenty-nine K antigen serotypes were demonstrated in V. parahaemolyticus isolates, and another 40% were untypable. The modal antibiotic resistance pattern for each species included five drugs. Only 12% of the V. parahaemolyticus strains were Kanagawa positive, and 10% elicited fluid accumulation in ligated rabbit ileal loops. All of the 7 V. alginolyticus strains and 94 (70%) of the V. parahaemolyticus strains tested killed mice when inoculated intraperitoneally.(ABSTRACT TRUNCATED AT 250 WORDS)

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Selected References

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  1. Bauer A. W., Kirby W. M., Sherris J. C., Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966 Apr;45(4):493–496. [PubMed] [Google Scholar]
  2. Baumann P., Baumann L., Mandel M. Taxonomy of marine bacteria: the genus Beneckea. J Bacteriol. 1971 Jul;107(1):268–294. doi: 10.1128/jb.107.1.268-294.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bockemühl J., Triemer A. Ecology and epidemiology of Vibrio parahaemolyticus on the coast of Togo. Bull World Health Organ. 1974;51(4):353–360. [PMC free article] [PubMed] [Google Scholar]
  4. Colwell R. R. Polyphasic taxonomy of the genus vibrio: numerical taxonomy of Vibrio cholerae, Vibrio parahaemolyticus, and related Vibrio species. J Bacteriol. 1970 Oct;104(1):410–433. doi: 10.1128/jb.104.1.410-433.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gjerde J., Böe B. Isolation and characterization of Vibrio alginolyticus and Vibrio parahaemolyticus from the Norwegian coastal environment. Acta Vet Scand. 1981;22(3-4):331–343. doi: 10.1186/BF03548658. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Joseph S. W., Colwell R. R., Kaper J. B. Vibrio parahaemolyticus and related halophilic Vibrios. Crit Rev Microbiol. 1982;10(1):77–124. doi: 10.3109/10408418209113506. [DOI] [PubMed] [Google Scholar]
  7. Joseph S. W., DeBell R. M., Brown W. P. In vitro response to chloramphenicol, tetracycline, ampicillin, gentamicin, and beta-lactamase production by halophilic Vibrios from human and environmental sources. Antimicrob Agents Chemother. 1978 Feb;13(2):244–248. doi: 10.1128/aac.13.2.244. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kampelmacher E. H., van Noorle Jansen L. M., Mossel D. A., Groen F. J. A survey of the occurrence of Vibrio parahaemolyticus and V. alginolyticus on mussels and oysters and in estuarine waters in the Netherlands. J Appl Bacteriol. 1972 Sep;35(3):431–438. doi: 10.1111/j.1365-2672.1972.tb03719.x. [DOI] [PubMed] [Google Scholar]
  9. Kaneko T., Colwell R. R. Adsorption of Vibrio parahaemolyticus onto chitin and copepods. Appl Microbiol. 1975 Feb;29(2):269–274. doi: 10.1128/am.29.2.269-274.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kaneko T., Colwell R. R. Distribution of Vibrio parahaemolyticus and related organisms in the Atlantic Ocean off South Carolina and Georgia. Appl Microbiol. 1974 Dec;28(6):1009–1017. doi: 10.1128/am.28.6.1009-1017.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kaneko T., Colwell R. R. Incidence of Vibrio parahaemolyticus in Chesapeake Bay. Appl Microbiol. 1975 Aug;30(2):251–257. doi: 10.1128/am.30.2.251-257.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Larsen J. L., Farid A. F., Dalsgaard I. A comprehensive study of environmental and human pathogenic Vibrio alginolyticus strains. Zentralbl Bakteriol Mikrobiol Hyg A. 1981 Dec;251(2):213–222. [PubMed] [Google Scholar]
  13. Mandel M., Igambi L., Bergendahl J., Dodson M. L., Jr, Scheltgen E. Correlation of melting temperature and cesium chloride buoyant density of bacterial deoxyribonucleic acid. J Bacteriol. 1970 Feb;101(2):333–338. doi: 10.1128/jb.101.2.333-338.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Nair G. B., Abraham M., Natarajan R. Distribution of Vibrio parahaemolyticus in finfish harvested from Porto Novo (S. India) environs: a seasonal study. Can J Microbiol. 1980 Nov;26(11):1264–1269. doi: 10.1139/m80-211. [DOI] [PubMed] [Google Scholar]
  15. SAKAZAKI R., IWANAMI S., FUKUMI H. STUDIES ON THE ENTEROPATHOGENIC, FACULTATIVELY HALOPHILIC BACTERIA, VIBRIO PARAHAEMOLYTICUS. I. MORPHOLOGICAL, CULTURAL AND BIOCHEMICAL PROPERTIES AND ITS TAXONOMICAL POSITION. Jpn J Med Sci Biol. 1963 Aug;16:161–188. doi: 10.7883/yoken1952.16.161. [DOI] [PubMed] [Google Scholar]
  16. Sakazaki R. Proposal of Vibrio alginolyticus for the biotype 2 of Vibrio parahaemolyticus. Jpn J Med Sci Biol. 1968 Oct;21(5):359–362. doi: 10.7883/yoken1952.21.359. [DOI] [PubMed] [Google Scholar]
  17. Schandevyl P., Van Dyck E., Piot P. Halophilic Vibrio species from seafish in Senegal. Appl Environ Microbiol. 1984 Jul;48(1):236–238. doi: 10.1128/aem.48.1.236-238.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Spira W. M., Goepfert J. M. Bacillus cereus-induced fluid accumulation in rabbit ileal loops. Appl Microbiol. 1972 Sep;24(3):341–348. doi: 10.1128/am.24.3.341-348.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Staley T. E., Colwell R. R. Polynucleotide sequence relationships among Japanese and American strains of Vibrio parahaemolyticus. J Bacteriol. 1973 Jun;114(3):916–927. doi: 10.1128/jb.114.3.916-927.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Stephen S., Vaz A. L., Chandrashekara I., Achyutha Rao K. N. Characterization of Vibrio alginolyticus (Beneckea alginolytica) isolated from the fauna of Arabian sea. Indian J Med Res. 1978 Jul;68:7–11. [PubMed] [Google Scholar]
  21. Zen-Yoji H., Le Clair R. A., Ota K., Montague T. S. Comparison of Vibrio parahaemolyticus cultures isolated in the United States with those isolated in Japan. J Infect Dis. 1973 Mar;127(3):237–241. doi: 10.1093/infdis/127.3.237. [DOI] [PubMed] [Google Scholar]

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