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. 1986 May;51(5):1124–1126. doi: 10.1128/aem.51.5.1124-1126.1986

Improved Agar Bottle Plate for Isolation of Methanogens or Other Anaerobes in a Defined Gas Atmosphere

Monique Hermann 1,†, Kenneth M Noll 1, Ralph S Wolfe 1,*
PMCID: PMC239021  PMID: 16347059

Abstract

A bottle plate for the cultivation of methanogens or other organisms in a defined pressurized-gas atmosphere was developed. The bottle provides the convenience of an agar streak plate, solves the problem of the water exudate from agar medium, and provides a convenient way of adding or sampling a defined gas atmosphere.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aranki A., Freter R. Use of anaerobic glove boxes for the cultivation of strictly anaerobic bacteria. Am J Clin Nutr. 1972 Dec;25(12):1329–1334. doi: 10.1093/ajcn/25.12.1329. [DOI] [PubMed] [Google Scholar]
  2. Balch W. E., Fox G. E., Magrum L. J., Woese C. R., Wolfe R. S. Methanogens: reevaluation of a unique biological group. Microbiol Rev. 1979 Jun;43(2):260–296. doi: 10.1128/mr.43.2.260-296.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Balch W. E., Wolfe R. S. New approach to the cultivation of methanogenic bacteria: 2-mercaptoethanesulfonic acid (HS-CoM)-dependent growth of Methanobacterium ruminantium in a pressureized atmosphere. Appl Environ Microbiol. 1976 Dec;32(6):781–791. doi: 10.1128/aem.32.6.781-791.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Braun M., Schoberth S., Gottschalk G. Enumeration of bacteria forming acetate from H2 and CO2 in anaerobic habitats. Arch Microbiol. 1979 Mar 12;120(3):201–204. doi: 10.1007/BF00423066. [DOI] [PubMed] [Google Scholar]
  5. Edwards T., McBride B. C. New method for the isolation and identification of methanogenic bacteria. Appl Microbiol. 1975 Apr;29(4):540–545. doi: 10.1128/am.29.4.540-545.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HUNGATE R. E. The anaerobic mesophilic cellulolytic bacteria. Bacteriol Rev. 1950 Mar;14(1):1–49. doi: 10.1128/br.14.1.1-49.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Harris J. E. GELRITE as an Agar Substitute for the Cultivation of Mesophilic Methanobacterium and Methanobrevibacter Species. Appl Environ Microbiol. 1985 Oct;50(4):1107–1109. doi: 10.1128/aem.50.4.1107-1109.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jones W. J., Whitman W. B., Fields R. D., Wolfe R. S. Growth and plating efficiency of methanococci on agar media. Appl Environ Microbiol. 1983 Jul;46(1):220–226. doi: 10.1128/aem.46.1.220-226.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Miller T. L., Wolin M. J. A serum bottle modification of the Hungate technique for cultivating obligate anaerobes. Appl Microbiol. 1974 May;27(5):985–987. doi: 10.1128/am.27.5.985-987.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Uffen R. L., Wolfe R. S. Anaerobic growth of purple nonsulfur bacteria under dark conditions. J Bacteriol. 1970 Oct;104(1):462–472. doi: 10.1128/jb.104.1.462-472.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]

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