Skip to main content
PMC home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1986 May;51(5):1127–1129. doi: 10.1128/aem.51.5.1127-1129.1986

Selective-enrichment procedure for isolation of Listeria monocytogenes from fecal and biologic specimens.

M P Doyle, J L Schoeni
PMCID: PMC239022  PMID: 3089147

Abstract

A selective-enrichment procedure (SEP) was developed to isolate Listeria monocytogenes from fecal and biologic specimens. This procedure was compared with direct plating with McBride listeria agar and 2-, 4-, and 8-week cold-enrichment procedures in recovering L. monocytogenes from mouse fecal, liver, and brain specimens. Although the SEP occasionally did not isolate the organism from specimens proved positive by the other procedures, the SEP isolated L. monocytogenes from about two and five times as many specimens as the cold-enrichment and direct-plating procedures, respectively.

Full text

PDF
1128

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BEARNS R. E., GIRARD K. F. On the isolation of Listeria monocytogenes from biological specimens. Am J Med Technol. 1959 Mar-Apr;25(2):120–126. [PubMed] [Google Scholar]
  2. Barza M. Listeriosis and milk. N Engl J Med. 1985 Feb 14;312(7):438–440. doi: 10.1056/NEJM198502143120710. [DOI] [PubMed] [Google Scholar]
  3. Bojsen-Moller J. Human listeriosis. Diagnostic, epidemiological and clinical studies. Acta Pathol Microbiol Scand B Microbiol Immunol. 1972;Suppl 229:1–157. [PubMed] [Google Scholar]
  4. Fleming D. W., Cochi S. L., MacDonald K. L., Brondum J., Hayes P. S., Plikaytis B. D., Holmes M. B., Audurier A., Broome C. V., Reingold A. L. Pasteurized milk as a vehicle of infection in an outbreak of listeriosis. N Engl J Med. 1985 Feb 14;312(7):404–407. doi: 10.1056/NEJM198502143120704. [DOI] [PubMed] [Google Scholar]
  5. Gray M. L., Killinger A. H. Listeria monocytogenes and listeric infections. Bacteriol Rev. 1966 Jun;30(2):309–382. doi: 10.1128/br.30.2.309-382.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Schlech W. F., 3rd, Lavigne P. M., Bortolussi R. A., Allen A. C., Haldane E. V., Wort A. J., Hightower A. W., Johnson S. E., King S. H., Nicholls E. S. Epidemic listeriosis--evidence for transmission by food. N Engl J Med. 1983 Jan 27;308(4):203–206. doi: 10.1056/NEJM198301273080407. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES