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. 1986 Oct;52(4):689–695. doi: 10.1128/aem.52.4.689-695.1986

Method for flow cytometric detection of Listeria monocytogenes in milk.

C W Donnelly, G J Baigent
PMCID: PMC239098  PMID: 3096202

Abstract

This report describes a method for the detection of Listeria monocytogenes in raw milk by flow cytometric analysis of fluorescently labeled bacterial populations. The use of immunofluorescence in combination with measures of DNA content by propidium iodide labeling and size by light scattering enabled specific identification of L. monocytogenes from Streptococcus faecalis, Streptococcus agalactiae, Streptococcus uberis, Staphylococcus epidermidis, and Staphylococcus hyicus. Additional specific resolution of L. monocytogenes populations was achieved through selective enrichment of raw milk in Listeria enrichment broth. These procedures should permit the rapid screening of milk and other food samples for L. monocytogenes and eliminate many of the short-comings associated with conventional fluorescent-antibody procedures.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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