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. 1986 Nov;52(5):1096–1100. doi: 10.1128/aem.52.5.1096-1100.1986

Manganese Oxidation by Spores and Spore Coats of a Marine Bacillus Species

Johannes P M de Vrind 2, Elisabeth W de Vrind-de Jong 2,*, Jan-Willem H de Voogt 2, Peter Westbroek 2, Fred C Boogerd 2, Reinhardt A Rosson 2
PMCID: PMC239179  PMID: 16347208

Abstract

Bacillus sp. strain SG-1 is a marine bacterial species isolated from a near-shore manganese sediment sample. Its mature dormant spores promote the oxidation of Mn2+ to MnO2. By quantifying the amounts of immobilized and oxidized manganese, it was established that bound manganese was almost instantaneously oxidized. When the final oxidation of manganese by the spores was partly inhibited by NaN3 or anaerobiosis, an equivalent decrease in manganese immobilization was observed. After formation of a certain amount of MnO2 by the spores, the oxidation rate decreased. A maximal encrustment was observed after which no further oxidation occurred. The oxidizing activity could be recovered by reduction of the MnO2 with hydroxylamine. Once the spores were encrusted, they could bind significant amounts of manganese, even when no oxidation occurred. Purified spore coat preparations oxidized manganese at the same rate as intact spores. During the oxidation of manganese in spore coat preparations, molecular oxygen was consumed and protons were liberated. The data indicate that a spore coat component promoted the oxidation of Mn2+ in a biologically catalyzed process, after adsorption of the ion to incipiently formed MnO2. Eventually, when large amounts of MnO2 were allowed to accumulate, the active sites were masked and further oxidation was prevented.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ali S. H., Stokes J. L. Stimulation of heterotrophic and autotrophic growth of Sphaerotilus discophorus by manganous ions. Antonie Van Leeuwenhoek. 1971;37(4):519–528. doi: 10.1007/BF02218522. [DOI] [PubMed] [Google Scholar]
  2. Burdige D. J., Nealson K. H. Microbial manganese reduction by enrichment cultures from coastal marine sediments. Appl Environ Microbiol. 1985 Aug;50(2):491–497. doi: 10.1128/aem.50.2.491-497.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ghiorse W. C., Ehrlich H. L. Electron transport components of the MnO2 reductase system and the location of the terminal reductase in a marine Bacillus. Appl Environ Microbiol. 1976 Jun;31(6):977–985. doi: 10.1128/aem.31.6.977-985.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Goldman R. C., Tipper D. J. Bacillus subtilis spore coats: complexity and purification of a unique polypeptide component. J Bacteriol. 1978 Sep;135(3):1091–1106. doi: 10.1128/jb.135.3.1091-1106.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. JANSSEN F. W., LUND A. J., ANDERSON L. E. Colorimetric assay for dipicolinic acid in bacterial spores. Science. 1958 Jan 3;127(3288):26–27. doi: 10.1126/science.127.3288.26. [DOI] [PubMed] [Google Scholar]
  6. Pandey N. K., Aronson A. I. Properties of the Bacillus subtilis spore coat. J Bacteriol. 1979 Mar;137(3):1208–1218. doi: 10.1128/jb.137.3.1208-1218.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Rosson R. A., Nealson K. H. Manganese binding and oxidation by spores of a marine bacillus. J Bacteriol. 1982 Aug;151(2):1027–1034. doi: 10.1128/jb.151.2.1027-1034.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Rosson R. A., Tebo B. M., Nealson K. H. Use of poisons in determination of microbial manganese binding rates in seawater. Appl Environ Microbiol. 1984 Apr;47(4):740–745. doi: 10.1128/aem.47.4.740-745.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Trimble R. B., Ehrlich H. L. Bacteriology of manganese nodules. IV. Induction of an MnO2-reductase system in a marine bacillus. Appl Microbiol. 1970 Jun;19(6):966–972. doi: 10.1128/am.19.6.966-972.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Warth A. D. Molecular structure of the bacterial spore. Adv Microb Physiol. 1978;17:1–45. doi: 10.1016/s0065-2911(08)60056-9. [DOI] [PubMed] [Google Scholar]
  11. de Vrind J. P., Boogerd F. C., de Vrind-de Jong E. W. Manganese reduction by a marine Bacillus species. J Bacteriol. 1986 Jul;167(1):30–34. doi: 10.1128/jb.167.1.30-34.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. van Veen W. L., Mulder E. G., Deinema M. H. The Sphaerotilus-Leptothrix group of bacteria. Microbiol Rev. 1978 Jun;42(2):329–356. doi: 10.1128/mr.42.2.329-356.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

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