Skip to main content
PMC home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1983 Jul;46(1):81–89. doi: 10.1128/aem.46.1.81-89.1983

Plasmid transfer in Pediococcus spp.: intergeneric and intrageneric transfer of pIP501.

C F Gonzalez, B S Kunka
PMCID: PMC239271  PMID: 6311098

Abstract

Transfer of the broad-host-range resistance plasmid pIP501 from Streptococcus faecalis to Pediococcus pentosaceus and Pediococcus acidilactici occurred between cells immobilized on nitrocellulose filters in the presence of DNase. Expression of the pIP501-linked erythromycin and chloramphenicol resistance determinants was observed in transconjugants. Intrageneric transfer of pIP501 from a P. pentosaceus donor to various pediococcal recipients occurred at frequencies of 10(-4) to 10(-7) transconjugants per input donor cell. Intergeneric transfer of plasmid pIP501 from P. pentosaceus to S. faecalis, Streptococcus sanguis (Challis), and Streptococcus lactis was observed. Similar mating experiments showed no evidence for the transfer of the broad-host-range R-plasmid pAM beta 1 to Pediococcus spp. recipients.

Full text

PDF
82

Images in this article

85Image
on p.85
87Image
on p.87

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. COSTER E., WHITE H. R. FURTHER STUDIES OF THE GENUS PEDIOCOCCUS. J Gen Microbiol. 1964 Oct;37:15–31. doi: 10.1099/00221287-37-1-15. [DOI] [PubMed] [Google Scholar]
  2. Clewell D. B. Plasmids, drug resistance, and gene transfer in the genus Streptococcus. Microbiol Rev. 1981 Sep;45(3):409–436. doi: 10.1128/mr.45.3.409-436.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Currier T. C., Nester E. W. Isolation of covalently closed circular DNA of high molecular weight from bacteria. Anal Biochem. 1976 Dec;76(2):431–441. doi: 10.1016/0003-2697(76)90338-9. [DOI] [PubMed] [Google Scholar]
  4. DEIBEL R. H., WILSON G. D., NIVEN C. F., Jr Microbiology of meat curing. IV. A lyophilized Pediococcus cerevisiae starter culture for fermented sausage. Appl Microbiol. 1961 May;9:239–243. doi: 10.1128/am.9.3.239-243.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Evans R. P., Jr, Macrina F. L. Streptococcal R plasmid pIP501: endonuclease site map, resistance determinant location, and construction of novel derivatives. J Bacteriol. 1983 Jun;154(3):1347–1355. doi: 10.1128/jb.154.3.1347-1355.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. GUNTHER H. L., WHITE H. R. The cultural and physiological characters of the Pediococci. J Gen Microbiol. 1961 Oct;26:185–197. doi: 10.1099/00221287-26-2-185. [DOI] [PubMed] [Google Scholar]
  7. Gibson E. M., Chace N. M., London S. B., London J. Transfer of plasmid-mediated antibiotic resistance from streptococci to lactobacilli. J Bacteriol. 1979 Jan;137(1):614–619. doi: 10.1128/jb.137.1.614-619.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gonzalez C. F., Vidaver A. K. Bacteriocin, plasmid and pectolytic diversity in Pseudomonas cepacia of clinical and plant origin. J Gen Microbiol. 1979 Jan;110(1):161–170. doi: 10.1099/00221287-110-1-161. [DOI] [PubMed] [Google Scholar]
  9. Hansen J. B., Abiko Y., Curtiss R., 3rd Characterization of the Streptococcus mutans plasmid pva318 cloned into Escherichia coli. Infect Immun. 1981 Mar;31(3):1034–1043. doi: 10.1128/iai.31.3.1034-1043.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hansen J. B., Olsen R. H. Isolation of large bacterial plasmids and characterization of the P2 incompatibility group plasmids pMG1 and pMG5. J Bacteriol. 1978 Jul;135(1):227–238. doi: 10.1128/jb.135.1.227-238.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hershfield V. Plasmids mediating multiple drug resistance in group B streptococcus: transferability and molecular properties. Plasmid. 1979 Jan;2(1):137–149. doi: 10.1016/0147-619x(79)90012-x. [DOI] [PubMed] [Google Scholar]
  12. Horodniceanu T., Bouanchaud D. H., Bieth G., Chabbert Y. A. R plasmids in Streptococcus agalactiae (group B). Antimicrob Agents Chemother. 1976 Nov;10(5):795–801. doi: 10.1128/aac.10.5.795. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Klaenhammer T. R., McKay L. L., Baldwin K. A. Improved lysis of group N streptococci for isolation and rapid characterization of plasmid deoxyribonucleic acid. Appl Environ Microbiol. 1978 Mar;35(3):592–600. doi: 10.1128/aem.35.3.592-600.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Leblanc D. J., Lee L. N. Rapid screening procedure for detection of plasmids in streptococci. J Bacteriol. 1979 Dec;140(3):1112–1115. doi: 10.1128/jb.140.3.1112-1115.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Macrina F. L., Kopecko D. J., Jones K. R., Ayers D. J., McCowen S. M. A multiple plasmid-containing Escherichia coli strain: convenient source of size reference plasmid molecules. Plasmid. 1978 Jun;1(3):417–420. doi: 10.1016/0147-619x(78)90056-2. [DOI] [PubMed] [Google Scholar]
  16. McKay L. L., Baldwin K. A., Walsh P. M. Conjugal transfer of genetic information in group N streptococci. Appl Environ Microbiol. 1980 Jul;40(1):84–89. doi: 10.1128/aem.40.1.84-91.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mundt J. O., Beattie W. G., Wieland F. R. Pediococci residing on plants. J Bacteriol. 1969 Jun;98(3):938–942. doi: 10.1128/jb.98.3.938-942.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. PEDERSON C. S. The genus Pediococcus. Bacteriol Rev. 1949 Dec;13(4):225–232. doi: 10.1128/br.13.4.225-232.1949. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Smith M. D., Guild W. R. Improved method for conjugative transfer by filter mating of Streptococcus pneumoniae. J Bacteriol. 1980 Oct;144(1):457–459. doi: 10.1128/jb.144.1.457-459.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Smith M. D., Shoemaker N. B., Burdett V., Guild W. R. Transfer of plasmids by conjugation in Streptococcus pneumonias. Plasmid. 1980 Jan;3(1):70–79. doi: 10.1016/s0147-619x(80)90035-9. [DOI] [PubMed] [Google Scholar]
  21. Vandenbergh P. A., Syed S. A., Gonzalez C. F., Loesche W. J., Olsen R. H. Plasmid content of some oral microorganisms isolated from subgingival plaque. J Dent Res. 1982 Mar;61(3):497–501. doi: 10.1177/00220345820610031201. [DOI] [PubMed] [Google Scholar]
  22. Weisblum B., Holder S. B., Halling S. M. Deoxyribonucleic acid sequence common to staphylococcal and streptococcal plasmids which specify erythromycin resistance. J Bacteriol. 1979 Jun;138(3):990–998. doi: 10.1128/jb.138.3.990-998.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES