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. 1983 Jul;46(1):260–263. doi: 10.1128/aem.46.1.260-263.1983

Formation, Fusion, and Regeneration of Protoplasts from Wild-Type and Auxotrophic Strains of the White Rot Basidiomycete Phanerochaete chrysosporium

Michael H Gold 1, Therese M Cheng 1, Margaret Alic 1
PMCID: PMC239297  PMID: 16346345

Abstract

A preparation of two commercial enzymes was used to liberate protoplasts from 16-h-old mycelium of Phanerochaete chrysosporium. Regeneration frequencies of up to 5% were attained when the protoplasts were plated in a medium containing 10% sorbose and 3% agar. Fusion of protoplasts from different auxotrophic strains in polyethylene glycol-Ca2+ produced heterokaryons. Separation of the heterokaryons into their constituent homokaryotic strains could be effected through protoplast release and formation of colonies on regeneration agar.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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