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. 1983 Oct;46(4):785–792. doi: 10.1128/aem.46.4.785-792.1983

Influence of Calcium and Manganese on Dechaining of Lactobacillus bulgaricus†

Catherine T Wright 1, Todd R Klaenhammer 1
PMCID: PMC239468  PMID: 16346396

Abstract

The events responsible for the transition of Lactobacillus bulgaricus 1243-F from long filamentous chains to short bacilloid rods were examined in a cation-depleted liquid medium. In the presence of magnesium only, cells grew as long chains of unseparated cells. The addition of 100 μM to 1 mM calcium or manganese to this medium resulted in the dechaining of these cells to short bacilloid rods. Fe2+, Zn2+, Co2+, and Cu2+ failed to induce dechaining. Induction of calcium and manganese dechaining functioned under controlled pH maintained at 5.0 and 6.0 but not at pH 7.0. This was consistent with a previous report showing failure in synthesis of dechaining enzymes by L. bulgaricus under pH conditions approaching alkalinity (S. K. Rhee and M. Y. Pack, J. Bacteriol. 144:865-868, 1980). We conclude that under pH conditions which permit synthesis of dechaining enzymes, calcium and manganese are necessary for dechaining activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barber F. W., Frazier W. C. Dissociants of Lactobacilli. J Bacteriol. 1945 Dec;50(6):637–649. [PMC free article] [PubMed] [Google Scholar]
  2. Chatterjee A. N., Wong W., Young F. E., Gilpin R. W. Isolation and characterization of a mutant of Staphylococcus aureus deficient in autolytic activity. J Bacteriol. 1976 Mar;125(3):961–967. doi: 10.1128/jb.125.3.961-967.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Coyette J., Shockman G. D. Some properties of the autolytic N-acetylmuramidase of Lactobacillus acidophilus. J Bacteriol. 1973 Apr;114(1):34–41. doi: 10.1128/jb.114.1.34-41.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fan D. P. Autolysin(s) of Bacillus subtilis as dechaining enzyme. J Bacteriol. 1970 Aug;103(2):494–499. doi: 10.1128/jb.103.2.494-499.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Forsberg C. W., Rogers H. J. Characterization of Bacillus licheniformis 6346 mutants which have altered lytic enzyme activities. J Bacteriol. 1974 May;118(2):358–368. doi: 10.1128/jb.118.2.358-368.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. GRULA E. A., GRULA M. M. Cell division in a species of Erwinia III. Reversal of inhibition of cell division caused by D-amino acids, penicillin, and ultraviolet light. J Bacteriol. 1962 May;83:981–988. doi: 10.1128/jb.83.5.981-988.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Herbold D. R., Glaser L. Bacillus subtilis N-acetylmuramic acid L-alanine amidase. J Biol Chem. 1975 Mar 10;250(5):1676–1682. [PubMed] [Google Scholar]
  8. Herbold D. R., Glaser L. Interaction of N-acetylmuramic acid L-alanine amidase with cell wall polymers. J Biol Chem. 1975 Sep 25;250(18):7231–7238. [PubMed] [Google Scholar]
  9. Higgins M. L., Coyette J., Shockman G. D. Sites of cellular autolysis in Lactobacillus acidophilus. J Bacteriol. 1973 Dec;116(3):1375–1382. doi: 10.1128/jb.116.3.1375-1382.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hurst A., Stubbs J. M. Electron microscopic study of membranes and walls of bacteria and changes occurring during growth initiation. J Bacteriol. 1969 Mar;97(3):1466–1479. doi: 10.1128/jb.97.3.1466-1479.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Höltje J. V., Tomasz A. Specific recognition of choline residues in the cell wall teichoic acid by the N-acetylmuramyl-L-alanine amidase of Pneumococcus. J Biol Chem. 1975 Aug 10;250(15):6072–6076. [PubMed] [Google Scholar]
  12. Klaenhammer T. R., Kleeman E. G. Growth Characteristics, Bile Sensitivity, and Freeze Damage in Colonial Variants of Lactobacillus acidophilus. Appl Environ Microbiol. 1981 Jun;41(6):1461–1467. doi: 10.1128/aem.41.6.1461-1467.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kojima M., Suda S., Hotta S., Hamada K., Suganuma A. Necessity of calcium ion for cell division in Lactobacillus bifidus. J Bacteriol. 1970 Nov;104(2):1010–1013. doi: 10.1128/jb.104.2.1010-1013.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rhee S. K., Pack M. Y. Effect of environmental pH on chain length of lactobacillus bulgaricus. J Bacteriol. 1980 Dec;144(3):865–868. doi: 10.1128/jb.144.3.865-868.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sozzi T., Maret R., Poulin J. M. Study of plating efficiency of bacteriophages of thermophilic lactic acid bacteria on different media. Appl Environ Microbiol. 1976 Jul;32(1):131–137. doi: 10.1128/aem.32.1.131-137.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wolf-Watz H., Normark S. Evidence for a role of N-acetylmuramyl-L-alanine amidase in septum separation in Escherichia coli. J Bacteriol. 1976 Nov;128(2):580–586. doi: 10.1128/jb.128.2.580-586.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

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