Abstract
Synthetic sewage containing phenol, acetone, and alkanols plus 4-chlorophenol or a mixture of isomeric chlorophenols is completely degraded by a defined mixed culture with Pseudomonas sp. strain B13 as a chlorocatechol-dissimilating member of the community. Total degradation of the organic carbon was indicated by release of stoichiometric amounts of chloride and low content of dissolved organic carbon in the cell-free effluents. During adaptation to high loads of chlorophenols the initial meta-cleavage activity was completely replaced by ortho-cleavage activity of type I and II. In the fully acclimated culture, hybrid strains such as Alcaligenes sp. strain A7-2 were detected, which are more competitive than Pseudomonas sp. strain B13 with respect to chlorophenol degradation.
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- Dorn E., Hellwig M., Reineke W., Knackmuss H. J. Isolation and characterization of a 3-chlorobenzoate degrading pseudomonad. Arch Microbiol. 1974;99(1):61–70. doi: 10.1007/BF00696222. [DOI] [PubMed] [Google Scholar]
- Dorn E., Knackmuss H. J. Chemical structure and biodegradability of halogenated aromatic compounds. Substituent effects on 1,2-dioxygenation of catechol. Biochem J. 1978 Jul 15;174(1):85–94. doi: 10.1042/bj1740085. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dorn E., Knackmuss H. J. Chemical structure and biodegradability of halogenated aromatic compounds. Two catechol 1,2-dioxygenases from a 3-chlorobenzoate-grown pseudomonad. Biochem J. 1978 Jul 15;174(1):73–84. doi: 10.1042/bj1740073. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Evans W. C., Smith B. S., Moss P., Fernley H. N. Bacterial metabolism of 4-chlorophenoxyacetate. Biochem J. 1971 May;122(4):509–517. doi: 10.1042/bj1220509. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Janke D., Fritsche W. Dechlorierung von 4-Chlorphenol nach extradioler Ringspaltung durch Pseudomonas putida. Z Allg Mikrobiol. 1979;19(2):139–141. doi: 10.1002/jobm.3630190210. [DOI] [PubMed] [Google Scholar]
- Klecka G. M., Gibson D. T. Inhibition of catechol 2,3-dioxygenase from Pseudomonas putida by 3-chlorocatechol. Appl Environ Microbiol. 1981 May;41(5):1159–1165. doi: 10.1128/aem.41.5.1159-1165.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Knackmuss H. J., Hellwig M. Utilization and cooxidation of chlorinated phenols by Pseudomonas sp. B 13. Arch Microbiol. 1978 Apr 27;117(1):1–7. doi: 10.1007/BF00689343. [DOI] [PubMed] [Google Scholar]
- STOCKS P. K., MCCLESKEY C. S. IDENTITY OF THE PINK-PIGMENTED METHANOL-OXIDIZING BACTERIA AS VIBRIO EXTORQUENS. J Bacteriol. 1964 Oct;88:1065–1070. doi: 10.1128/jb.88.4.1065-1070.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schmidt E., Knackmuss H. J. Chemical structure and biodegradability of halogenated aromatic compounds. Conversion of chlorinated muconic acids into maleoylacetic acid. Biochem J. 1980 Oct 15;192(1):339–347. doi: 10.1042/bj1920339. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schwien U., Schmidt E. Improved degradation of monochlorophenols by a constructed strain. Appl Environ Microbiol. 1982 Jul;44(1):33–39. doi: 10.1128/aem.44.1.33-39.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vandenbergh P. A., Olsen R. H., Colaruotolo J. F. Isolation and genetic characterization of bacteria that degrade chloroaromatic compounds. Appl Environ Microbiol. 1981 Oct;42(4):737–739. doi: 10.1128/aem.42.4.737-739.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]