Abstract
Studies on the characterization of salivarian trypanosomes are at present developing rapidly and difficulties in communication tend to arise because of different procedures in the different laboratories concerned. Terms can be divided into two classes, those that are purely operational and simply describe the laboratory history of the organism, and those that imply characterization. Of the Linnaean taxa, the section Salivaria and the four subgenera thereof, Duttonella, Nannomonas, Pycnomonas, and Trypanozoon, are useful at the present time but lower taxa are often unsatisfactorily defined. Characters such as the clinical course of infection, which have been used for subspeciation, are being found to correlate with such characters as isoenzyme patterns, antigenic make-up and DNA constitution.
Serial passage of organisms has serious disadvantages and so reference collections are primarily of stabilates—cryopreserved suspensions of living organisms. Methods for the preparation of antigens, antisera, and isoenzymes are briefly noted.
Conventions for the documentation of reference collections are proposed, in particular that the designation of materials should consist of two components, one signifying the primary isolation and the other the particular derived material used. The requirements for rapid information retrieval and global cataloguing of materials are considered.
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Selected References
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- Afchain D., Fruit J., Petitprez A., Van Meirvenne N., Le Ray D., Bout D., Capron A. Antigenic analysis of a variant specific component of Trypanosoma Brucei Brucei I -- Immunochemical purification by affinity chromatography. Pathol Biol (Paris) 1976 Nov;24(9):615–617. [PubMed] [Google Scholar]
- Godfrey D. G., Kilgour V. Enzyme electrophoresis in characterizing the causative organism of Gambian trypanosomiasis. Trans R Soc Trop Med Hyg. 1976;70(3):219–224. doi: 10.1016/0035-9203(76)90043-2. [DOI] [PubMed] [Google Scholar]
- Gray A. R. Antigenic variation in a strain of Trypanosoma brucei transmitted by Glossina morsitans and G. palpalis. J Gen Microbiol. 1965 Nov;41(2):195–214. doi: 10.1099/00221287-41-2-195. [DOI] [PubMed] [Google Scholar]
- Kilgour V., Godfrey D. G., Na'isa B. K. Isoenzymes of two aminotransferases among Trypanosoma vivax in Nigerian cattle. Ann Trop Med Parasitol. 1975 Sep;69(3):329–335. doi: 10.1080/00034983.1975.11687016. [DOI] [PubMed] [Google Scholar]
- Kilgour V., Godfrey D. G. Species-characteristic isoenzymes of two aminotransferases in trypanosomes. Nat New Biol. 1973 Jul 18;244(133):69–70. doi: 10.1038/newbio244069a0. [DOI] [PubMed] [Google Scholar]
- Le Ray D., Afchain D., Jadin J., Capron A., Fameree L. Interrelations immuno-taxonomiques de T. brucei, T. rhodesiense et T. gambiense. Ann Parasitol Hum Comp. 1971 Sep-Oct;46(5):523–531. [PubMed] [Google Scholar]
- Le Ray D. Structures antigeniques de Trypsnosoma brucei (Protozoa, Kinetoplastida). Analyse immunoélectrophorétique et étude comparative. Ann Soc Belg Med Trop. 1975;55(3):129–311. [PubMed] [Google Scholar]
- Roberts C. J. The lack of infectivity to cattle of a strain of Trypanosoma simiae transmitted by Glossina morsitans and G. tachinoides. Ann Trop Med Parasitol. 1971 Sep;65(3):319–326. doi: 10.1080/00034983.1971.11686760. [DOI] [PubMed] [Google Scholar]
- Van Meirvenne N., Janssens P. G., Magnus E. Antigenic variation in syringe passaged populations of Trypanosoma (Trypanozoon) brucei. 1. Rationalization of the experimental approach. Ann Soc Belg Med Trop. 1975;55(1):1–23. [PubMed] [Google Scholar]
- Van Meirvenne N., Janssens P. G., Magnus E., Lumsden W. H., Herbert W. J. Antigenic variation in syringe passaged populations of Trypanosoma (Trypanozoon) brucei. II. Comparative studies on two antigenic-type collections. Ann Soc Belg Med Trop. 1975;55(1):25–30. [PubMed] [Google Scholar]
- van Meirvenne N., Maginus E., Janssens P. G. The effect of normal human serum on trypanosomes of distinct antigenic type (ETat 1 to 12) isolated from a strain of Trypanosoma brucei rhodesiense. Ann Soc Belg Med Trop. 1976;56(1):55–63. [PubMed] [Google Scholar]