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. 1991 Feb;65(2):1002–1006. doi: 10.1128/jvi.65.2.1002-1006.1991

Human fetal Schwann cells support JC virus multiplication.

J G Assouline 1, E O Major 1
PMCID: PMC239848  PMID: 1846179

Abstract

The human papovavirus JC virus (JCV), the etiologic agent of progressive multifocal leukoencephalopathy, displays a narrow host range for growth, preferentially infecting oligodendrocytes, the myelin-producing cells of the central nervous system. In tissue culture, human fetal brain cells have been used for JCV propagation because of their ability to support JCV virion production. In this study, we evidence that a human fetal cell type derived from the peripheral nervous system can be productively infected with JCV. Schwann cells, the cell type responsible for myelination in the peripheral nervous system, support the expression of JCV T antigen and JCV DNA replication. However, viral proteins and DNA replication were not detected either in dorsal root ganglion neurons or fibroblasts. These results extend the host range of JCV to include another cell of the glial lineage whose function is myelin formation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aksamit A. J., Sever J. L., Major E. O. Progressive multifocal leukoencephalopathy: JC virus detection by in situ hybridization compared with immunohistochemistry. Neurology. 1986 Apr;36(4):499–504. doi: 10.1212/wnl.36.4.499. [DOI] [PubMed] [Google Scholar]
  2. Assouline J. G., Pantazis N. J. Detection of a nerve growth factor receptor on fetal human Schwann cells in culture: absence of the receptor on fetal human astrocytes. Brain Res Dev Brain Res. 1989 Jan 1;45(1):1–14. doi: 10.1016/0165-3806(89)90002-3. [DOI] [PubMed] [Google Scholar]
  3. Dörries K., Johnson R. T., ter Meulen V. Detection of polyoma virus DNA in PML-brain tissue by (in situ) hybridization. J Gen Virol. 1979 Jan;42(1):49–57. doi: 10.1099/0022-1317-42-1-49. [DOI] [PubMed] [Google Scholar]
  4. Elder G. A., Major E. O. Early appearance of type II astrocytes in developing human fetal brain. Brain Res. 1988 Jul 1;470(1):146–150. doi: 10.1016/0165-3806(88)90211-8. [DOI] [PubMed] [Google Scholar]
  5. Feigenbaum L., Khalili K., Major E., Khoury G. Regulation of the host range of human papovavirus JCV. Proc Natl Acad Sci U S A. 1987 Jun;84(11):3695–3698. doi: 10.1073/pnas.84.11.3695. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gorman C. M., Moffat L. F., Howard B. H. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol Cell Biol. 1982 Sep;2(9):1044–1051. doi: 10.1128/mcb.2.9.1044. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
  8. Major E. O., Mourrain P., Cummins C. JC virus-induced owl monkey glioblastoma cells in culture: biological properties associated with the viral early gene product. Virology. 1984 Jul 30;136(2):359–367. doi: 10.1016/0042-6822(84)90172-7. [DOI] [PubMed] [Google Scholar]
  9. Major E. O., Vacante D. A. Human fetal astrocytes in culture support the growth of the neurotropic human polyomavirus, JCV. J Neuropathol Exp Neurol. 1989 Jul;48(4):425–436. doi: 10.1097/00005072-198907000-00004. [DOI] [PubMed] [Google Scholar]
  10. Major E. O., Vacante D. A., Traub R. G., London W. T., Sever J. L. Owl monkey astrocytoma cells in culture spontaneously produce infectious JC virus which demonstrates altered biological properties. J Virol. 1987 May;61(5):1435–1441. doi: 10.1128/jvi.61.5.1435-1441.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mandl C., Walker D. L., Frisque R. J. Derivation and characterization of POJ cells, transformed human fetal glial cells that retain their permissivity for JC virus. J Virol. 1987 Mar;61(3):755–763. doi: 10.1128/jvi.61.3.755-763.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Martin J. D., Padgett B. L., Walker D. L. Characterization of tissue culture-induced heterogeneity in DNAs of independent isolates of JC virus. J Gen Virol. 1983 Oct;64(Pt 10):2271–2280. doi: 10.1099/0022-1317-64-10-2271. [DOI] [PubMed] [Google Scholar]
  13. Mázló M., Tariska I. Morphological demonstration of the first phase of polyomavirus replication in oligodendroglia cells of human brain in progressive multifocal leukoencephalopathy (PML). Acta Neuropathol. 1980;49(2):133–143. doi: 10.1007/BF00690753. [DOI] [PubMed] [Google Scholar]
  14. Nakamura S., Tourtellotte W. W., Shapshak P., Darvish M. [The detection of papovavirus nucleotide sequences in cortical neurons of a patient with progressive multifocal leucoencephalopathy (PML) by in situ hybridization]. Rinsho Shinkeigaku. 1985 Oct;25(10):1148–1151. [PubMed] [Google Scholar]
  15. Padgett B. L., Rogers C. M., Walker D. L. JC virus, a human polyomavirus associated with progressive multifocal leukoencephalopathy: additional biological characteristics and antigenic relationships. Infect Immun. 1977 Feb;15(2):656–662. doi: 10.1128/iai.15.2.656-662.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Padgett B. L., Walker D. L., ZuRhein G. M., Eckroade R. J., Dessel B. H. Cultivation of papova-like virus from human brain with progressive multifocal leucoencephalopathy. Lancet. 1971 Jun 19;1(7712):1257–1260. doi: 10.1016/s0140-6736(71)91777-6. [DOI] [PubMed] [Google Scholar]
  17. Stoner G. L., Ryschkewitsch C. F., Walker D. L., Soffer D., Webster H. D. A monoclonal antibody to SV40 large T-antigen labels a nuclear antigen in JC virus-transformed cells and in progressive multifocal leukoencephalopathy (PML) brain infected with JC virus. J Neuroimmunol. 1988 Mar;17(4):331–345. doi: 10.1016/0165-5728(88)90124-5. [DOI] [PubMed] [Google Scholar]
  18. Tada H., Lashgari M., Rappaport J., Khalili K. Cell type-specific expression of JC virus early promoter is determined by positive and negative regulation. J Virol. 1989 Jan;63(1):463–466. doi: 10.1128/jvi.63.1.463-466.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wigdahl B., Guyton R. A., Sarin P. S. Human immunodeficiency virus infection of the developing human nervous system. Virology. 1987 Aug;159(2):440–445. doi: 10.1016/0042-6822(87)90483-1. [DOI] [PubMed] [Google Scholar]
  20. Wiley C. A., Grafe M., Kennedy C., Nelson J. A. Human immunodeficiency virus (HIV) and JC virus in acquired immune deficiency syndrome (AIDS) patients with progressive multifocal leukoencephalopathy. Acta Neuropathol. 1988;76(4):338–346. doi: 10.1007/BF00686970. [DOI] [PubMed] [Google Scholar]

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