Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1991 Mar;65(3):1600–1604. doi: 10.1128/jvi.65.3.1600-1604.1991

Recurring theme of changes in the transcriptional control region of BK virus during adaptation to cell culture.

R Rubinstein 1, B C Schoonakker 1, E H Harley 1
PMCID: PMC239945  PMID: 1847478

Abstract

BK virus, when cloned directly from human urine, shows no amplification in the transcriptional control region, unlike cell culture-passaged strains, but possesses an additional sequence element. To confirm our proposal that this represents the archetypal in vivo form of the virus, we passaged this BK virus through cell culture. Changes in the transcriptional control region occurred as early as the first passage and were characterized in all cases by a deletion followed by amplification events.

Full text

PDF
1604

Images in this article

1601Image
on p.1601
1601Image
on p.1601

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chauhan S., Lecatsas G., Harley E. H. Genome analysis of BK (WW) viral DNA cloned directly from human urine. Intervirology. 1984;22(3):170–176. doi: 10.1159/000149547. [DOI] [PubMed] [Google Scholar]
  2. Dalrymple S. A., Beemon K. L. BK virus T antigens induce kidney carcinomas and thymoproliferative disorders in transgenic mice. J Virol. 1990 Mar;64(3):1182–1191. doi: 10.1128/jvi.64.3.1182-1191.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Del Vecchio A. M., Steinman R. A., Ricciardi R. P. An element of the BK virus enhancer required for DNA replication. J Virol. 1989 Apr;63(4):1514–1524. doi: 10.1128/jvi.63.4.1514-1524.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Frisque R. J., Bream G. L., Cannella M. T. Human polyomavirus JC virus genome. J Virol. 1984 Aug;51(2):458–469. doi: 10.1128/jvi.51.2.458-469.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gardner S. D., Field A. M., Coleman D. V., Hulme B. New human papovavirus (B.K.) isolated from urine after renal transplantation. Lancet. 1971 Jun 19;1(7712):1253–1257. doi: 10.1016/s0140-6736(71)91776-4. [DOI] [PubMed] [Google Scholar]
  6. Hara K., Oya Y., Kinoshita H., Taguchi F., Yogo Y. Sequence reiteration required for the efficient growth of BK virus. J Gen Virol. 1986 Nov;67(Pt 11):2555–2559. doi: 10.1099/0022-1317-67-11-2555. [DOI] [PubMed] [Google Scholar]
  7. Hearing P., Shenk T. The adenovirus type 5 E1A transcriptional control region contains a duplicated enhancer element. Cell. 1983 Jul;33(3):695–703. doi: 10.1016/0092-8674(83)90012-0. [DOI] [PubMed] [Google Scholar]
  8. Loeber G., Dörries K. DNA rearrangements in organ-specific variants of polyomavirus JC strain GS. J Virol. 1988 May;62(5):1730–1735. doi: 10.1128/jvi.62.5.1730-1735.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Markowitz R. B., Dynan W. S. Binding of cellular proteins to the regulatory region of BK virus DNA. J Virol. 1988 Sep;62(9):3388–3398. doi: 10.1128/jvi.62.9.3388-3398.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Markowitz R. B., Tolbert S., Dynan W. S. Promoter evolution in BK virus: functional elements are created at sequence junctions. J Virol. 1990 May;64(5):2411–2415. doi: 10.1128/jvi.64.5.2411-2415.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pagnani M., Negrini M., Reschiglian P., Corallini A., Balboni P. G., Scherneck S., Macino G., Milanesi G., Barbanti-Brodano G. Molecular and biological properties of BK virus-IR, a BK virus variant isolated from a human tumor. J Virol. 1986 Aug;59(2):500–505. doi: 10.1128/jvi.59.2.500-505.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Pater A., Pater M. M., Chang L. S., Slawin K., Di Mayorca G. Multiple origins of the complementary defective genomes of RF and origin proximal sequences of GS, two human papovavirus isolates. Virology. 1983 Dec;131(2):426–436. doi: 10.1016/0042-6822(83)90509-3. [DOI] [PubMed] [Google Scholar]
  13. Rosenthal N., Kress M., Gruss P., Khoury G. BK viral enhancer element and a human cellular homolog. Science. 1983 Nov 18;222(4625):749–755. doi: 10.1126/science.6314501. [DOI] [PubMed] [Google Scholar]
  14. Rubinstein R., Harley E. H. BK virus DNA cloned directly from human urine confirms an archetypal structure for the transcriptional control region. Virus Genes. 1989 Mar;2(2):157–165. doi: 10.1007/BF00315259. [DOI] [PubMed] [Google Scholar]
  15. Rubinstein R., Pare N., Harley E. H. Structure and function of the transcriptional control region of nonpassaged BK virus. J Virol. 1987 May;61(5):1747–1750. doi: 10.1128/jvi.61.5.1747-1750.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Seif I., Khoury G., Dhar R. The genome of human papovavirus BKV. Cell. 1979 Dec;18(4):963–977. doi: 10.1016/0092-8674(79)90209-5. [DOI] [PubMed] [Google Scholar]
  18. Shinagawa M., Matsuda A., Ishiyama T., Goto H., Sato G. A rapid and simple method for preparation of adenovirus DNA from infected cells. Microbiol Immunol. 1983;27(9):817–822. doi: 10.1111/j.1348-0421.1983.tb00638.x. [DOI] [PubMed] [Google Scholar]
  19. Sugimoto C., Hara K., Taguchi F., Yogo Y. Growth efficiency of naturally occurring BK virus variants in vivo and in vitro. J Virol. 1989 Jul;63(7):3195–3199. doi: 10.1128/jvi.63.7.3195-3199.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Tada H., Lashgari M., Rappaport J., Khalili K. Cell type-specific expression of JC virus early promoter is determined by positive and negative regulation. J Virol. 1989 Jan;63(1):463–466. doi: 10.1128/jvi.63.1.463-466.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Tavis J. E., Walker D. L., Gardner S. D., Frisque R. J. Nucleotide sequence of the human polyomavirus AS virus, an antigenic variant of BK virus. J Virol. 1989 Feb;63(2):901–911. doi: 10.1128/jvi.63.2.901-911.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Watanabe S., Yoshiike K. Evolutionary changes of transcriptional control region in a minute-plaque viable deletion mutant of BK virus. J Virol. 1986 Aug;59(2):260–266. doi: 10.1128/jvi.59.2.260-266.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Yang R. C., Wu R. Comparative study of papovavirus DNA: BKV(MM), BKV(WT) and SV40. Nucleic Acids Res. 1979 Oct 10;7(3):651–668. doi: 10.1093/nar/7.3.651. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES