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. 1992 Dec;66(12):7168–7175. doi: 10.1128/jvi.66.12.7168-7175.1992

Antiviral effects of a thiol protease inhibitor on foot-and-mouth disease virus.

L G Kleina 1, M J Grubman 1
PMCID: PMC240409  PMID: 1331517

Abstract

The thiol protease inhibitor E-64 specifically blocks autocatalytic activity of the leader protease of foot-and-mouth disease virus (FMDV) and interferes with cleavage of the structural protein precursor in an in vitro translation assay programmed with virion RNA. Experiments with FMDV-infected cells and E-64 or a membrane-permeable analog, E-64d, have confirmed these results and demonstrated interference in virus assembly, causing a reduction in virus yield. In addition, there is a lag in the appearance of virus-induced cellular morphologic alterations, a delay in cleavage of host cell protein p220 and in shutoff of host protein synthesis, and a decrease in viral protein and RNA synthesis. The implications of using E-64-based compounds as potential antiviral agents for FMDV are discussed.

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