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. 1991 Jan;65(1):42–50. doi: 10.1128/jvi.65.1.42-50.1991

Binding of SL3-3 enhancer factor 1 transcriptional activators to viral and chromosomal enhancer sequences.

A Thornell 1, B Hallberg 1, T Grundström 1
PMCID: PMC240487  PMID: 1985206

Abstract

Interactions between SL3-3 enhancer factor 1 (SEF1) proteins and the enhancer of the murine leukemia virus SL3-3 were analyzed. SEF1 proteins were found to interact with two different DNA sequences within the DNA repeat region of the enhancer; these two motifs cooperated in enhancing initiation of transcription in T lymphocytes. Using an electrophoretic mobility shift assay, we identified nucleotides that are important for the SEF1 binding, and we deduced a sequence, 5'-TTTGCGGTTA/T-3' with highly improved binding of SEF1 proteins. We show that many different SEF1 binding sequences exist in the transcription control regions of different viral and cellular genes. The results indicate a general role of SEF1 proteins in T-cell gene expression.

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Selected References

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