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. 1991 Jun;65(6):2798–2806. doi: 10.1128/jvi.65.6.2798-2806.1991

Cooperative assembly of simian virus 40 T-antigen hexamers on functional halves of the replication origin.

R E Parsons 1, J E Stenger 1, S Ray 1, R Welker 1, M E Anderson 1, P Tegtmeyer 1
PMCID: PMC240896  PMID: 1851855

Abstract

The cofactor ATP stimulates the formation of T-antigen double hexamers on the simian virus 40 core origin of replication (I. A. Mastrangelo, P. V. C. Hough, J. S. Wall, M. Dodson, F. B. Dean, and J. Horwitz, Nature [London] 338:658-662, 1989). We report here the pathway for the assembly of hexamers and double hexamers on the core origin. ATP triggers the cooperative assembly of hexamers on the early and late halves of the origin even when they are completely isolated. Hexamer assembly nucleates at T-antigen recognition pentanucleotides in the early half of the origin. In intact origins, assembly of the first hexamer on the early half of the origin cooperatively stimulates the assembly of a second hexamer on the adjacent late half of the origin. Thus, monomer-monomer and hexamer-hexamer interactions of T antigen, allosterically activated by ATP, constitute two distinct types of cooperative interaction with the origin. Finally, we show that the assembly of T-antigen hexamers on isolated half origins leads to the same array of structural changes that T antigen induces in intact origins. We conclude that the origin is divided into complementary halves that each promote the assembly of functional T-antigen hexamers.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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