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. 1992 Mar;66(3):1746–1751. doi: 10.1128/jvi.66.3.1746-1751.1992

Effect of zinc ions on the biochemical behavior of simian virus 40 small-t antigen expressed in bacteria.

R Goswami 1, B Turk 1, K Enderle 1, A Howe 1, K Rundell 1
PMCID: PMC240925  PMID: 1310775

Abstract

The simian virus 40 small-t antigen contains 10 cysteine residues, 6 of which are organized in two CysXCysXXCys clusters. Mutation of individual Cys residues in the two clusters or mutation of specific residues found between these clusters causes pronounced instability of the protein in animal cells. Protein instability correlates with failure of the bacterially expressed mutant proteins to bind zinc ions, an interaction which allows purification of large amounts of small-t antigen in monomeric form.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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