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. 1992 May;66(5):2605–2610. doi: 10.1128/jvi.66.5.2605-2610.1992

Heterologous recombination in the double-stranded RNA bacteriophage phi 6.

L Mindich 1, X Qiao 1, S Onodera 1, P Gottlieb 1, J Strassman 1
PMCID: PMC241013  PMID: 1560519

Abstract

Bacteriophage phi 6 contains three double-stranded RNA genomic segments. We have constructed a virus with an insertion of a kanamycin resistance gene in genomic RNA segment M. The virus forms small, turbid plaques, and its genome is unstable. Virus from a single plaque contained from about 0.1 to 10% large clear-plaque forms of the virus; these were usually missing the kanamycin resistance gene, and in many cases, the resulting segment M was larger or smaller than its normal size. Sequence analysis of the genomic RNA of the apparent deletions showed that they were formed by recombination events between segment M and either segment S or L. These heterologous recombination events resulted in the loss of the kanamycin resistance gene from segment M and the replacement of the 3' end of segment M with the 3' end of segment S or L. Although the 3' ends of the single-stranded RNA transcripts of the genomic segments appear to have extensive secondary structure, the sequences at the 3' ends are not involved in the specificity of genomic packaging.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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