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. 1992 May;66(5):3168–3171. doi: 10.1128/jvi.66.5.3168-3171.1992

Evidence for zinc binding by two structural proteins of Plodia interpunctella granulosis virus.

C J Funk 1, R A Consigli 1
PMCID: PMC241081  PMID: 1560542

Abstract

Workers in our laboratory previously reported the possibility of cation involvement in the in vitro dissociation of the Plodia interpunctella granulosis virus nucleocapsids (K. A. Tweeten, L. A. Bulla, Jr., and R. A. Consigli, J. Virol. 33:866-876, 1980; M. E. Wilson and R. A. Consigli, Virology 143:516-525, 1985). The current study found zinc associated with both granulosis virus nucleocapsids and granulin by atomic absorption analysis. A blotting assay with 65Zn2+ specifically identified the radioactive cation as binding to two viral structural proteins, granulin and VP12. These findings indicate that zinc may have a critical role in maintaining virus stability.

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Selected References

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  1. Berg J. M. Potential metal-binding domains in nucleic acid binding proteins. Science. 1986 Apr 25;232(4749):485–487. doi: 10.1126/science.2421409. [DOI] [PubMed] [Google Scholar]
  2. Bess J. W., Jr, Powell P. J., Issaq H. J., Schumack L. J., Grimes M. K., Henderson L. E., Arthur L. O. Tightly bound zinc in human immunodeficiency virus type 1, human T-cell leukemia virus type I, and other retroviruses. J Virol. 1992 Feb;66(2):840–847. doi: 10.1128/jvi.66.2.840-847.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Birk H. W., Koepsell H. Reaction of monoclonal antibodies with plasma membrane proteins after binding on nitrocellulose: renaturation of antigenic sites and reduction of nonspecific antibody binding. Anal Biochem. 1987 Jul;164(1):12–22. doi: 10.1016/0003-2697(87)90360-5. [DOI] [PubMed] [Google Scholar]
  4. Brady J. N., Winston V. D., Consigli R. A. Dissociation of polyoma virus by the chelation of calcium ions found associated with purified virions. J Virol. 1977 Sep;23(3):717–724. doi: 10.1128/jvi.23.3.717-724.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Consigli R. A., Russell D. L., Wilson M. E. The biochemistry and molecular biology of the granulosis virus that infects Plodia interpunctella. Curr Top Microbiol Immunol. 1986;131:69–101. doi: 10.1007/978-3-642-71589-1_5. [DOI] [PubMed] [Google Scholar]
  6. Durham A. C., Hendry D. A., Von Wechmar M. B. Does calcium ion binding control plant virus disassembly? Virology. 1977 Apr;77(2):524–533. doi: 10.1016/0042-6822(77)90478-0. [DOI] [PubMed] [Google Scholar]
  7. Green L. M., Berg J. M. Retroviral nucleocapsid protein-metal ion interactions: folding and sequence variants. Proc Natl Acad Sci U S A. 1990 Aug;87(16):6403–6407. doi: 10.1073/pnas.87.16.6403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gupte S. S., Olson J. W., Ruyechan W. T. The major herpes simplex virus type-1 DNA-binding protein is a zinc metalloprotein. J Biol Chem. 1991 Jun 25;266(18):11413–11416. [PubMed] [Google Scholar]
  9. Hogle J., Kirchhausen T., Harrison S. C. Divalent cation sites in tomato bushy stunt virus. Difference maps at 2-9 A resolution. J Mol Biol. 1983 Nov 25;171(1):95–100. doi: 10.1016/s0022-2836(83)80315-5. [DOI] [PubMed] [Google Scholar]
  10. Karabash Iu A., Karpov A. E. Aktyvatsiia latentnoho virusu iadernoho poledrozu v huseni kil'chastoho shovkopriada Malacosoma neustria. Mikrobiol Zh. 1973 Nov-Dec;35(6):781–782. [PubMed] [Google Scholar]
  11. Ludlow J. W., Consigli R. A. Localization of calcium on the polyomavirus VP1 capsid protein. J Virol. 1987 Sep;61(9):2934–2937. doi: 10.1128/jvi.61.9.2934-2937.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Maeda S., Kamita S. G., Kataoka H. The basic DNA-binding protein of Bombyx mori nuclear polyhedrosis virus: the existence of an additional arginine repeat. Virology. 1991 Feb;180(2):807–810. doi: 10.1016/0042-6822(91)90096-t. [DOI] [PubMed] [Google Scholar]
  13. Maruyama K., Mikawa T., Ebashi S. Detection of calcium binding proteins by 45Ca autoradiography on nitrocellulose membrane after sodium dodecyl sulfate gel electrophoresis. J Biochem. 1984 Feb;95(2):511–519. doi: 10.1093/oxfordjournals.jbchem.a134633. [DOI] [PubMed] [Google Scholar]
  14. Rohrmann G. F. Polyhedrin structure. J Gen Virol. 1986 Aug;67(Pt 8):1499–1513. doi: 10.1099/0022-1317-67-8-1499. [DOI] [PubMed] [Google Scholar]
  15. Russell R. L., Rohrmann G. F. The p6.5 gene region of a nuclear polyhedrosis virus of Orgyia pseudotsugata: DNA sequence and transcriptional analysis of four late genes. J Gen Virol. 1990 Mar;71(Pt 3):551–560. doi: 10.1099/0022-1317-71-3-551. [DOI] [PubMed] [Google Scholar]
  16. Schiff L. A., Nibert M. L., Co M. S., Brown E. G., Fields B. N. Distinct binding sites for zinc and double-stranded RNA in the reovirus outer capsid protein sigma 3. Mol Cell Biol. 1988 Jan;8(1):273–283. doi: 10.1128/mcb.8.1.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schiff L. A., Nibert M. L., Fields B. N. Characterization of a zinc blotting technique: evidence that a retroviral gag protein binds zinc. Proc Natl Acad Sci U S A. 1988 Jun;85(12):4195–4199. doi: 10.1073/pnas.85.12.4195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sehnke P. C., Mason A. M., Hood S. J., Lister R. M., Johnson J. E. A "zinc-finger"-type binding domain in tobacco streak virus coat protein. Virology. 1989 Jan;168(1):48–56. doi: 10.1016/0042-6822(89)90402-9. [DOI] [PubMed] [Google Scholar]
  19. Summers M. D. Electron microscopic observations on granulosis virus entry, uncoating and replication processes during infection of the midgut cells of Trichoplusia ni. J Ultrastruct Res. 1971 Jun;35(5):606–625. doi: 10.1016/s0022-5320(71)80014-x. [DOI] [PubMed] [Google Scholar]
  20. Summers M. D., Smith G. E. Baculovirus structural polypeptides. Virology. 1978 Feb;84(2):390–402. doi: 10.1016/0042-6822(78)90257-x. [DOI] [PubMed] [Google Scholar]
  21. Tweeten K. A., Bulla L. A., Consigli R. A. Characterization of an extremely basic protein derived from granulosis virus nucleocapsids. J Virol. 1980 Feb;33(2):866–876. doi: 10.1128/jvi.33.2.866-876.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Tweeten K. A., Bulla L. A., Consigli R. A. Structural Polypeptides of the Granulosis Virus of Plodia interpunctella. J Virol. 1980 Feb;33(2):877–886. doi: 10.1128/jvi.33.2.877-886.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Tweeten K. A., Bulla L. A., Jr, Consigli R. A. Characterization of an alkaline protease associated with a granulosis virus of Plodia interpunctella. J Virol. 1978 Jun;26(3):703–711. [PMC free article] [PubMed] [Google Scholar]
  24. Tweeten K. A., Bulla L. A., Jr, Consigli R. A. Isolation and purification of a granulosis virus from infected larvae of the Indian meal moth, Plodia interpunctella. Appl Environ Microbiol. 1977 Sep;34(3):320–327. doi: 10.1128/aem.34.3.320-327.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Vallee B. L., Auld D. S. Zinc coordination, function, and structure of zinc enzymes and other proteins. Biochemistry. 1990 Jun 19;29(24):5647–5659. doi: 10.1021/bi00476a001. [DOI] [PubMed] [Google Scholar]
  26. Vallee B. L., Coleman J. E., Auld D. S. Zinc fingers, zinc clusters, and zinc twists in DNA-binding protein domains. Proc Natl Acad Sci U S A. 1991 Feb 1;88(3):999–1003. doi: 10.1073/pnas.88.3.999. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Vallee B. L., Galdes A. The metallobiochemistry of zinc enzymes. Adv Enzymol Relat Areas Mol Biol. 1984;56:283–430. doi: 10.1002/9780470123027.ch5. [DOI] [PubMed] [Google Scholar]
  28. Weiss S. A., Smith G. C., Vaughn J. L., Dougherty E. M., Tompkins G. J. Effect of aluminum chloride and zinc sulfate on Autographa california nuclear polyhedrosis virus (ACNPV) replication in cell culture. In Vitro. 1982 Nov;18(11):937–944. doi: 10.1007/BF02796350. [DOI] [PubMed] [Google Scholar]
  29. Wilson M. E., Mainprize T. H., Friesen P. D., Miller L. K. Location, transcription, and sequence of a baculovirus gene encoding a small arginine-rich polypeptide. J Virol. 1987 Mar;61(3):661–666. doi: 10.1128/jvi.61.3.661-666.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Yamashita M. M., Wesson L., Eisenman G., Eisenberg D. Where metal ions bind in proteins. Proc Natl Acad Sci U S A. 1990 Aug;87(15):5648–5652. doi: 10.1073/pnas.87.15.5648. [DOI] [PMC free article] [PubMed] [Google Scholar]

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