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. 1984 Aug;48(2):371–375. doi: 10.1128/aem.48.2.371-375.1984

Evaluation of m-T7 agar as a fecal coliform medium.

M W LeChevallier, P E Jakanoski, A K Camper, G A McFeters
PMCID: PMC241520  PMID: 6385849

Abstract

m-T7 agar, designed to improve recoveries of injured total coliforms, was evaluated for its effectiveness as a fecal coliform medium. The time and temperature of preincubation were found to be crucial to the optimal recovery of fetal coliforms. Isolation rates for fecal coliforms on m-T7 agar from sewage effluents were the highest when plates were preincubated at 37 degrees C for 8 h before transfer to 44.5 degrees C for 12 h. The medium was found to produce consistently higher fecal coliform counts than all the other methods tested. Recoveries were 3.1 times greater than the standard m-FC method and 1.7 times greater than the two-layer enrichment, temperature acclimation procedure. Verification rates for fecal coliforms isolated on m-T7 agar averaged 89.0%, whereas verification rates for m-FC agar averaged only 82.8%. Both media isolated similar fecal coliform populations. The advantages of a single medium, highly effective for the isolation of both total and fecal coliforms, are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bissonnette G. K., Jezeski J. J., McFeters G. A., Stuart D. G. Influence of environmental stress on enumeration of indicator bacteria from natural waters. Appl Microbiol. 1975 Feb;29(2):186–194. doi: 10.1128/am.29.2.186-194.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Braswell J. R., Hoadley A. W. Recovery of Escherichia coli from chlorinated secondary sewage. Appl Microbiol. 1974 Aug;28(2):328–329. doi: 10.1128/am.28.2.328-329.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Camper A. K., McFeters G. A. Chlorine injury and the enumeration of waterborne coliform bacteria. Appl Environ Microbiol. 1979 Mar;37(3):633–641. doi: 10.1128/aem.37.3.633-641.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dufour A. P., Strickland E. R., Cabelli V. J. Membrane filter method for enumerating Escherichia coli. Appl Environ Microbiol. 1981 May;41(5):1152–1158. doi: 10.1128/aem.41.5.1152-1158.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Green B. L., Clausen E. M., Litsky W. Two-temperature membrane filter method for enumerating fecal coliform bacteria from chlorinated effluents. Appl Environ Microbiol. 1977 Jun;33(6):1259–1264. doi: 10.1128/aem.33.6.1259-1264.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. LeChevallier M. W., Cameron S. C., McFeters G. A. New medium for improved recovery of coliform bacteria from drinking water. Appl Environ Microbiol. 1983 Feb;45(2):484–492. doi: 10.1128/aem.45.2.484-492.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lin S. D. Membrane filter method for recovery of fecal coliforms in chlorinated sewage effluents. Appl Environ Microbiol. 1976 Oct;32(4):547–552. doi: 10.1128/aem.32.4.547-552.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lin S. Evaluation of coliform tests for chlorinated secondary effluents. J Water Pollut Control Fed. 1973 Mar;45(1):498–506. [PubMed] [Google Scholar]
  9. McFeters G. A., Cameron S. C., LeChevallier M. W. Influence of diluents, media, and membrane filters on detection fo injured waterborne coliform bacteria. Appl Environ Microbiol. 1982 Jan;43(1):97–103. doi: 10.1128/aem.43.1.97-103.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Presswood W. G., Strong D. K. Modification of M-FC medium by eliminating rosolic acid. Appl Environ Microbiol. 1978 Jul;36(1):90–94. doi: 10.1128/aem.36.1.90-94.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rose R. E., Geldreich E. E., Litsky W. Improved membrane filter method for fecal coliform analysis. Appl Microbiol. 1975 Apr;29(4):532–536. doi: 10.1128/am.29.4.532-536.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Stuart D. G., McFeters G. A., Schillinger J. E. Membrane filter technique for the quantification of stressed fecal coliforms in the aquatic environment. Appl Environ Microbiol. 1977 Jul;34(1):42–46. doi: 10.1128/aem.34.1.42-46.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Zaske S. K., Dockins W. S., McFeters G. A. Cell envelope damage in Escherichia coli caused by short-term stress in water. Appl Environ Microbiol. 1980 Aug;40(2):386–390. doi: 10.1128/aem.40.2.386-390.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]

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