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. 1982 Feb;43(2):298–302. doi: 10.1128/aem.43.2.298-302.1982

Isolation of Plasmid DNA from Butyrivibrio fibrisolvens

Ronald M Teather 1
PMCID: PMC241822  PMID: 16345935

Abstract

A procedure based on successive precipitation of cell lysates with sodium dodecyl sulfate-NaCl and polyethylene glycol 6000 was developed which allows the isolation of plasmid DNA from Butyrivibrio fibrisolvens. A survey of B. fibrisolvens strains isolated from the bovine rumen showed that plasmids are a common feature of this species.

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Selected References

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  1. Aranki A., Freter R. Use of anaerobic glove boxes for the cultivation of strictly anaerobic bacteria. Am J Clin Nutr. 1972 Dec;25(12):1329–1334. doi: 10.1093/ajcn/25.12.1329. [DOI] [PubMed] [Google Scholar]
  2. BRYANT M. P., SMALL N. The anaerobic monotrichous butyric acid-producing curved rod-shaped bacteria of the rumen. J Bacteriol. 1956 Jul;72(1):16–21. doi: 10.1128/jb.72.1.16-21.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Barnes W. M. Plasmid detection and sizing in single colony lysates. Science. 1977 Jan 28;195(4276):393–394. doi: 10.1126/science.318764. [DOI] [PubMed] [Google Scholar]
  4. Caldwell D. R., Bryant M. P. Medium without rumen fluid for nonselective enumeration and isolation of rumen bacteria. Appl Microbiol. 1966 Sep;14(5):794–801. doi: 10.1128/am.14.5.794-801.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Eckhardt T. A rapid method for the identification of plasmid desoxyribonucleic acid in bacteria. Plasmid. 1978 Sep;1(4):584–588. doi: 10.1016/0147-619x(78)90016-1. [DOI] [PubMed] [Google Scholar]
  6. Guerry P., LeBlanc D. J., Falkow S. General method for the isolation of plasmid deoxyribonucleic acid. J Bacteriol. 1973 Nov;116(2):1064–1066. doi: 10.1128/jb.116.2.1064-1066.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HUNGATE R. E. The anaerobic mesophilic cellulolytic bacteria. Bacteriol Rev. 1950 Mar;14(1):1–49. doi: 10.1128/br.14.1.1-49.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hayward G. S., Smith M. G. The chromosome of bacteriophage T5. I. Analysis of the single-stranded DNA fragments by agarose gel electrophoresis. J Mol Biol. 1972 Feb 14;63(3):383–395. doi: 10.1016/0022-2836(72)90435-4. [DOI] [PubMed] [Google Scholar]
  9. Hughes C., Meynell G. G. Rapid screening for plasmid DNA. Mol Gen Genet. 1977 Mar 7;151(2):175–179. doi: 10.1007/BF00338692. [DOI] [PubMed] [Google Scholar]
  10. Laemmli U. K., Favre M. Maturation of the head of bacteriophage T4. I. DNA packaging events. J Mol Biol. 1973 Nov 15;80(4):575–599. doi: 10.1016/0022-2836(73)90198-8. [DOI] [PubMed] [Google Scholar]
  11. Sherratt D. J. Bacterial plasmids. Cell. 1974 Nov;3(3):189–195. doi: 10.1016/0092-8674(74)90130-5. [DOI] [PubMed] [Google Scholar]
  12. Teather R. M., Müller-Hill B., Abrutsch U., Aichele G., Overath P. Amplification of the lactose carrier protein in Escherichia coli using a plasmid vector. Mol Gen Genet. 1978 Feb 27;159(3):239–248. doi: 10.1007/BF00268260. [DOI] [PubMed] [Google Scholar]
  13. Telford J., Boseley P., Schaffner W., Birnstiel M. Novel screening procedure for recombinant plasmids. Science. 1977 Jan 28;195(4276):391–393. doi: 10.1126/science.318763. [DOI] [PubMed] [Google Scholar]
  14. Varel V. H., Bryant M. P., Holdeman L. V., Moore W. E. Isolation of ureolytic Peptostreptococcus productus from feces using defined medium; failure of common urease tests. Appl Microbiol. 1974 Oct;28(4):594–599. doi: 10.1128/am.28.4.594-599.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wozny M. A., Bryant M. P., Holdeman L. V., Moore W. E. Urease assay and urease-producing species of anaerobes in the bovine rumen and human feces. Appl Environ Microbiol. 1977 May;33(5):1097–1104. doi: 10.1128/aem.33.5.1097-1104.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

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