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. 1982 Jul;44(1):33–39. doi: 10.1128/aem.44.1.33-39.1982

Improved Degradation of Monochlorophenols by a Constructed Strain

Uwe Schwien 1,, Eberhard Schmidt 1,
PMCID: PMC241964  PMID: 16346066

Abstract

Pseudomonas sp. strain B13, a strain able to degrade 3-chlorobenzoate and, after prolonged adaptation (40 days), 4-chlorophenol, could transfer the ability to degrade chlorocatechols to a recipient, Alcaligenes sp. strain A7, which is able to grow with benzoate and phenol. Representative transconjugants, such as Alcaligenes sp. strain A7-2, were able to utilize all three isomeric chlorophenols; this property was not possessed by the donor or the recipient. The ability to grow readily with 4-chlorophenol may be attributable to a more rapid induction of phenol hydroxylase by Alcaligenes sp. strain A7-2 than by Pseudomonas sp. strain B13, a property which correlates with the greater level of resistance to chlorophenols shown by the transconjugant.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Davis D. H., Stanier R. Y., Doudoroff M., Mandel M. Taxonomic studies on some gram negative polarly flagellated "hydrogen bacteria" and related species. Arch Mikrobiol. 1970;70(1):1–13. doi: 10.1007/BF00691056. [DOI] [PubMed] [Google Scholar]
  2. Dorn E., Hellwig M., Reineke W., Knackmuss H. J. Isolation and characterization of a 3-chlorobenzoate degrading pseudomonad. Arch Microbiol. 1974;99(1):61–70. doi: 10.1007/BF00696222. [DOI] [PubMed] [Google Scholar]
  3. Dorn E., Knackmuss H. J. Chemical structure and biodegradability of halogenated aromatic compounds. Substituent effects on 1,2-dioxygenation of catechol. Biochem J. 1978 Jul 15;174(1):85–94. doi: 10.1042/bj1740085. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dorn E., Knackmuss H. J. Chemical structure and biodegradability of halogenated aromatic compounds. Two catechol 1,2-dioxygenases from a 3-chlorobenzoate-grown pseudomonad. Biochem J. 1978 Jul 15;174(1):73–84. doi: 10.1042/bj1740073. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Klecka G. M., Gibson D. T. Inhibition of catechol 2,3-dioxygenase from Pseudomonas putida by 3-chlorocatechol. Appl Environ Microbiol. 1981 May;41(5):1159–1165. doi: 10.1128/aem.41.5.1159-1165.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Knackmuss H. J., Hellwig M. Utilization and cooxidation of chlorinated phenols by Pseudomonas sp. B 13. Arch Microbiol. 1978 Apr 27;117(1):1–7. doi: 10.1007/BF00689343. [DOI] [PubMed] [Google Scholar]
  7. Mayfield C. I., Inniss W. E. A rapid, simple method for staining bacterial flagella. Can J Microbiol. 1977 Sep;23(9):1311–1313. doi: 10.1139/m77-198. [DOI] [PubMed] [Google Scholar]
  8. Reineke W., Knackmuss H. J. Hybrid pathway for chlorobenzoate metabolism in Pseudomonas sp. B13 derivatives. J Bacteriol. 1980 May;142(2):467–473. doi: 10.1128/jb.142.2.467-473.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. SCHMIDT K., LIAAENJENSEN S., SCHLEGEL H. G. DIE CAROTINOIDE DER THIORHODACEAE. I. OKENON ALS HAUPTEAROTINOID VON CHROMATIUM OKENII PERTY. Arch Mikrobiol. 1963 Aug 1;46:117–126. [PubMed] [Google Scholar]
  10. Schmidt E., Knackmuss H. J. Chemical structure and biodegradability of halogenated aromatic compounds. Conversion of chlorinated muconic acids into maleoylacetic acid. Biochem J. 1980 Oct 15;192(1):339–347. doi: 10.1042/bj1920339. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Tyler J. E., Finn R. K. Growth rates of a pseudomonad on 2,4-dichlorophenoxyacetic acid and 2,4-dichlorophenol. Appl Microbiol. 1974 Aug;28(2):181–184. doi: 10.1128/am.28.2.181-184.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

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