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. 1982 Dec;44(6):1318–1324. doi: 10.1128/aem.44.6.1318-1324.1982

Acetone and Butanol Production by Clostridium acetobutylicum in a Synthetic Medium

Frédéric Monot 1, Jean-René Martin 1, Henri Petitdemange 1, Robert Gay 1
PMCID: PMC242190  PMID: 16346149

Abstract

The effect of the component concentrations of a synthetic medium on acetone and butanol fermentation by Clostridium acetobutylicum ATCC 824 was investigated. Cell growth was dependent on the presence of Mg, Fe, and K in the medium. Mg and Mn had deleterious effects when in excess. Ammonium acetate in excess caused acid fermentation. The metabolism was composed of two phases: an acid phase and a solvent one. Low concentrations of glucose allowed the first phase only. The theoretical ratio of the conversion of glucose to solvents, which was 28 to 33%, was obtained with the following medium: MgSO4, 50 to 200 mg/liter; MnSO4, 0 to 20 mg/liter; KCl, 0.015 to 8 g/liter (an equivalent concentration of K+ was supplied in the form of KH2PO4 and K2HPO4); FeSO4, 1 to 50 mg/liter; ammonium acetate, 1.1 to 2.2 g/liter; para-aminobenzoic acid, 1 mg/liter; biotin, 0.01 mg/liter; glucose, 20 to 60 g/liter.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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