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. 1983 Jan;45(1):200–204. doi: 10.1128/aem.45.1.200-204.1983

Dual-Substrate Plate Diffusion Assay for Proteases

Thomas J Montville 1
PMCID: PMC242253  PMID: 6337548

Abstract

A plate diffusion assay for endopeptidases was developed. Proteases applied to plates containing 1% casein, 1% gelatin, and 1.5% agar caused distinct zones reminiscent of immunoprecipitation bands. The diameter of the zones was linearly proportional to the log of the enzyme activity applied over a range from 0.01 to greater than 100 IU/ml.

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Selected References

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