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. 1983 Feb;45(2):644–650. doi: 10.1128/aem.45.2.644-650.1983

Tn5-Induced Mutations in the Enterobacterial Phytopathogen Erwinia chrysanthemi†

Arun K Chatterjee 1, Kerry K Thurn 1, Dorothy A Feese 1
PMCID: PMC242338  PMID: 16346212

Abstract

Escherichia coli (2492/pJB4JI) matings with Erwinia chrysanthemi produced kanamycin resistant (Kmr) transconjugants, a majority of which were gentamicin sensitive (Gms). A small proportion (about 0.8%) of the Kmr Gms clones were either auxotrophic or failed to catabolize galacturonate (Gtu−). The R plasmid (pJB4JI) DNA was detected in the parent E. coli strain and in a Kmr Gmr transconjugant, but not in Kmr GmsE. chrysanthemi strains carrying Tn5-induced mutations. In Hfr crosses, Kmr (Tn5) was found linked with most mutations. A majority (>95%) of prototrophic recombinants were Kms, except for Leu+ and Arg+ recombinants which were 30 to 50% Kms. Spontaneous revertants were obtained for all markers except car, gtu, lys, thr, and trp. Prototrophic revertants, with the exception of Met+, Leu+, or His+ clones, were Kms. We conclude from both genetic and physical data that Tn5 transposed from pJB4JI into different sites on the chromosome of E. chrysanthemi.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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