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. 1983 May;45(5):1445–1452. doi: 10.1128/aem.45.5.1445-1452.1983

Transductional construction of a threonine-hyperproducing strain of Serratia marcescens: lack of feedback controls of three aspartokinases and two homoserine dehydrogenases.

S Komatsubara, M Kisumi, I Chibata
PMCID: PMC242483  PMID: 6307143

Abstract

To construct a threonine-hyperproducing strain of Serratia marcescens Sr41, the six regulatory mutations for three aspartokinases and two homoserine dehydrogenases were combined in a single strain by three transductional crosses. The constructed strain, T-1026, carried the lysC1 mutation leading to lack of feedback inhibition and repression of aspartokinase III, the thrA1(1) mutation desensitizing aspartokinase I to feedback inhibition, the thrA2(1) mutation releasing feedback inhibition of homoserine dehydrogenase I, the two hnr mutations derepressing aspartokinase I and homoserine dehydrogenase I, and the etr-1 mutation derepressing aspartokinase II and homoserine dehydrogenase II. The strain produced ca. 40 mg of threonine per ml of medium containing sucrose and urea. Furthermore, the productivity of strain T-1026 was compared with those of strains devoid of more than one of the six regulatory mutations.

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Selected References

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  1. Bachmann B. J., Low K. B. Linkage map of Escherichia coli K-12, edition 6. Microbiol Rev. 1980 Mar;44(1):1–56. doi: 10.1128/mr.44.1.1-56.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DAVIS B. D., MINGIOLI E. S. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. doi: 10.1128/jb.60.1.17-28.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Kisumi M., Komatsubara S., Chibata I. Enhancement of isoleucine hydroxamate-mediated growth inhibition and improvement of isoleucine-producing strains of Serratia marcescens. Appl Environ Microbiol. 1977 Dec;34(6):647–653. doi: 10.1128/aem.34.6.647-653.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kisumi M., Komatsubara S., Sugiura M., Chibata I. Isoleucine hydroxamate, an isoleucine antagonist. J Bacteriol. 1971 Sep;107(3):741–745. doi: 10.1128/jb.107.3.741-745.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Komatsubara S., Kisumi M., Chibata I. Participation of lysine-sensitive aspartokinase in threonine production by S-2-aminoethyl cysteine-resistant mutants of Serratia marcescens. Appl Environ Microbiol. 1979 Nov;38(5):777–782. doi: 10.1128/aem.38.5.777-782.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Komatsubara S., Kisumi M., Chibata I. Threonine production by ethionine-resistant mutants of Serratia marcescens. Appl Environ Microbiol. 1983 May;45(5):1437–1444. doi: 10.1128/aem.45.5.1437-1444.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Komatsubara S., Kisumi M., Chibata I. Transductional construction of a threonine-producing strain of Serratia marcescens. Appl Environ Microbiol. 1979 Dec;38(6):1045–1051. doi: 10.1128/aem.38.6.1045-1051.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Komatsubara S., Kisumi M., Chibata I. Transductional construction of an isoleucine-producing strain of Serratia marcescens. J Gen Microbiol. 1980 Jul;119(1):51–61. doi: 10.1099/00221287-119-1-51. [DOI] [PubMed] [Google Scholar]
  9. Komatsubara S., Kisumi M., Murata K., Chibata I. Threonine production by regulatory mutants of Serratia marcescens. Appl Environ Microbiol. 1978 May;35(5):834–840. doi: 10.1128/aem.35.5.834-840.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Komatsubara S., Murata K., Kisumi M., Chibata I. Threonine degradation by Serratia marcescens. J Bacteriol. 1978 Aug;135(2):318–323. doi: 10.1128/jb.135.2.318-323.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Matsumoto H., Hosogaya S., Suzuki K., Tazaki T. Arginine gene cluster of Serratia marcescens. Jpn J Microbiol. 1975 Feb;19(1):35–44. doi: 10.1111/j.1348-0421.1975.tb00845.x. [DOI] [PubMed] [Google Scholar]
  13. Matsumoto H., Tazaki T., Hosogaya S. A generalized transducing phage of Serratia marcescens. Jpn J Microbiol. 1973 Nov;17(6):473–479. doi: 10.1111/j.1348-0421.1973.tb00933.x. [DOI] [PubMed] [Google Scholar]
  14. Thèze J., Saint-Girons I. Threonine locus of Escherichia coli K-12: genetic structure and evidence for an operon. J Bacteriol. 1974 Jun;118(3):990–998. doi: 10.1128/jb.118.3.990-998.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Umbarger H. E. Amino acid biosynthesis and its regulation. Annu Rev Biochem. 1978;47:532–606. doi: 10.1146/annurev.bi.47.070178.002533. [DOI] [PubMed] [Google Scholar]
  16. Weiner R. M., Voll M. J., Cook T. M. Nalidixic acid for enrichment of auxotrophs in cultures of Salmonella typhimurium. Appl Microbiol. 1974 Oct;28(4):579–581. doi: 10.1128/am.28.4.579-581.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

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