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. 1983 May;45(5):1622–1632. doi: 10.1128/aem.45.5.1622-1632.1983

Bacterioplankton in Antarctic Ocean Waters During Late Austral Winter: Abundance, Frequency of Dividing Cells, and Estimates of Production

R B Hanson 1, David Shafer 1, Theresa Ryan 1, Daniel H Pope 1, H Kenneth Lowery 1
PMCID: PMC242509  PMID: 16346297

Abstract

Bacterioplankton productivity in Antarctic waters of the eastern South Pacific Ocean and Drake Passage was estimated by direct counts and frequency of dividing cells (FDC). Total bacterioplankton assemblages were enumerated by epifluorescent microscopy. The experimentally determined relationship between in situ FDC and the potential instantaneous growth rate constant (μ) is best described by the regression equation ln μ = 0.081 FDC − 3.73. In the eastern South Pacific Ocean, bacterioplankton abundance (2 × 105 to 3.5 × 105 cells per ml) and FDC (11%) were highest at the Polar Front (Antarctic Convergence). North of the Subantarctic Front, abundance and FDC were between 1 × 105 to 2 × 105 cells per ml and 3 to 5%, respectively, and were vertically homogeneous to a depth of 600 m. In Drake Passage, abundance (10 × 105 cells per ml) and FDC (16%) were highest in waters south of the Polar Front and near the sea ice. Subantarctic waters in Drake Passage contained 4 × 105 cells per ml with 4 to 5% FDC. Instantaneous growth rate constants ranged between 0.029 and 0.088 h−1. Using estimates of potential μ and measured standing stocks, we estimated productivity to range from 0.62 μg of C per liter · day in the eastern South Pacific Ocean to 17.1 μg of C per liter · day in the Drake Passage near the sea ice.

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Selected References

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  1. Buckley J. R., Gammelsrød T., Johannessen J. A., Johannessen O. M., Røed L. P. Upwelling: oceanic structure at the edge of the arctic ice pack in winter. Science. 1979 Jan 12;203(4376):165–167. doi: 10.1126/science.203.4376.165. [DOI] [PubMed] [Google Scholar]
  2. Christian R. R., Hanson R. B., Newell S. Y. Comparison of methods for measurement of bacterial growth rates in mixed batch cultures. Appl Environ Microbiol. 1982 May;43(5):1160–1165. doi: 10.1128/aem.43.5.1160-1165.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Donachie W. D., Begg K. J., Vicente M. Cell length, cell growth and cell division. Nature. 1976 Nov 25;264(5584):328–333. doi: 10.1038/264328a0. [DOI] [PubMed] [Google Scholar]
  4. Donachie W. D. Relationship between cell size and time of initiation of DNA replication. Nature. 1968 Sep 7;219(5158):1077–1079. doi: 10.1038/2191077a0. [DOI] [PubMed] [Google Scholar]
  5. Fuhrman J. A., Azam F. Bacterioplankton secondary production estimates for coastal waters of british columbia, antarctica, and california. Appl Environ Microbiol. 1980 Jun;39(6):1085–1095. doi: 10.1128/aem.39.6.1085-1095.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hagström A., Larsson U., Hörstedt P., Normark S. Frequency of dividing cells, a new approach to the determination of bacterial growth rates in aquatic environments. Appl Environ Microbiol. 1979 May;37(5):805–812. doi: 10.1128/aem.37.5.805-812.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hobbie J. E., Daley R. J., Jasper S. Use of nuclepore filters for counting bacteria by fluorescence microscopy. Appl Environ Microbiol. 1977 May;33(5):1225–1228. doi: 10.1128/aem.33.5.1225-1228.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Karl D. M. Simultaneous rates of ribonucleic Acid and deoxyribonucleic Acid syntheses for estimating growth and cell division of aquatic microbial communities. Appl Environ Microbiol. 1981 Nov;42(5):802–810. doi: 10.1128/aem.42.5.802-810.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kirchman D., Sigda J., Kapuscinski R., Mitchell R. Statistical analysis of the direct count method for enumerating bacteria. Appl Environ Microbiol. 1982 Aug;44(2):376–382. doi: 10.1128/aem.44.2.376-382.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Newell S. Y., Christian R. R. Frequency of dividing cells as an estimator of bacterial productivity. Appl Environ Microbiol. 1981 Jul;42(1):23–31. doi: 10.1128/aem.42.1.23-31.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Woldringh C. L. Morphological analysis of nuclear separation and cell division during the life cycle of Escherichia coli. J Bacteriol. 1976 Jan;125(1):248–257. doi: 10.1128/jb.125.1.248-257.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Wright R. T. Measurement and significance of specific activity in the heterotrophic bacteria of natural waters. Appl Environ Microbiol. 1978 Aug;36(2):297–305. doi: 10.1128/aem.36.2.297-305.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Zimmermann R., Iturriaga R., Becker-Birck J. Simultaneous determination of the total number of aquatic bacteria and the number thereof involved in respiration. Appl Environ Microbiol. 1978 Dec;36(6):926–935. doi: 10.1128/aem.36.6.926-935.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

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