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. 1977 Aug;34(2):207–221. doi: 10.1128/aem.34.2.207-221.1977

Nose, throat, and fecal flora of beagle dogs housed in "locked" or "open" environments.

E Balish, D Cleven, J Brown, C E Yale
PMCID: PMC242622  PMID: 907342

Abstract

The microbial flora of the nose, throat, and feces of male beagle dogs housed in a "locked environment" (i.e. confined to germfree-style isolators and supplied with sterile food, air, and water) or an open environment were assessed between 26 and 30 months into the study. Forty-five genera and 170 different species or types of microorganisms were cultured from the nose, throat, and feces of the beagles. Clostridia, eubacteria, corynebacteria, bacteroides, lactobacilli, and anaerobic, gram-positive cocci accounted for most of the microbial diversity in the flora. Some of the facultative anaerobes, especially streptococci and lactobacilli (in feces), occurred in numbers that were comparable to the most numerous anaerobic species. Confinement to the locked environment resulted in an increased diversity of microorganisms in the flora, but the total microbial counts did not increase to any great extent. Even with the increased diversity of bacteria in the flora of confined dogs, some bacteria seemed to favor certain areas of the gastrointestinal tract over others. The increased diversity of bacteria observed in these confined dogs may pose some infectious disease problems for other mammals (including humans) that may be confined to a locked, ultra-clean environment for a prolonged period of time.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arank A., Syed S. A., Kenney E. B., Freter R. Isolation of anaerobic bacteria from human gingiva and mouse cecum by means of a simplified glove box procedure. Appl Microbiol. 1969 Apr;17(4):568–576. doi: 10.1128/am.17.4.568-576.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Aranki A., Freter R. Use of anaerobic glove boxes for the cultivation of strictly anaerobic bacteria. Am J Clin Nutr. 1972 Dec;25(12):1329–1334. doi: 10.1093/ajcn/25.12.1329. [DOI] [PubMed] [Google Scholar]
  3. Balish E., Brown J. F., Wilkins T. D. Transparent plastic incubator for the anaerobic glove box. Appl Environ Microbiol. 1977 Mar;33(3):525–527. doi: 10.1128/aem.33.3.525-527.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Balish E., Shih C. N., Yale C. E., Mandel A. D. Effect of 30 months in a locked environment on the microbial flora of dogs. Aviat Space Environ Med. 1977 May;48(5):424–431. [PubMed] [Google Scholar]
  5. Balish E., Shih C. N., Yale C. E., Mandel A. D. Effect of a prolonged stay in a locked environment on the microbial flora in dogs. Aerosp Med. 1974 Nov;45(11):1248–1254. [PubMed] [Google Scholar]
  6. Bornside G. H., Cohn I., Jr The normal microbial flora: comparative bacterial flora of animals and man. Am J Dig Dis. 1965 Oct;10(10):844–852. doi: 10.1007/BF02236093. [DOI] [PubMed] [Google Scholar]
  7. Brennan P. C., Simkins R. C. Throat flora of a closed colony of beagles. Proc Soc Exp Biol Med. 1970 Jun;134(2):566–569. doi: 10.3181/00379727-134-34836. [DOI] [PubMed] [Google Scholar]
  8. CLAPPER W. E., MEADE G. H. NORMAL FLORA OF THE NOSE, THROAT, AND LOWER INTESTINE OF DOGS. J Bacteriol. 1963 Mar;85:643–648. doi: 10.1128/jb.85.3.643-648.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Davis C. P., Cleven D., Balish E., Yale C. E. Bacterial association in the gastrointestinal tract of beagle dogs. Appl Environ Microbiol. 1977 Aug;34(2):194–206. doi: 10.1128/aem.34.2.194-206.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Decelle J. G., Taylor G. R. Autoflora in the upper respiratory tract of Apollo astronauts. Appl Environ Microbiol. 1976 Nov;32(5):659–665. doi: 10.1128/aem.32.5.659-665.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hansen S. L., Stewart B. J. Comparison of API and Minitek to Center for Disease Control methods for the biochemical characterization of anaerobes. J Clin Microbiol. 1976 Sep;4(3):227–231. doi: 10.1128/jcm.4.3.227-231.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Holdeman L. V., Good I. J., Moore W. E. Human fecal flora: variation in bacterial composition within individuals and a possible effect of emotional stress. Appl Environ Microbiol. 1976 Mar;31(3):359–375. doi: 10.1128/aem.31.3.359-375.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Luckey T. D. Potential microbic shock in manned aerospace systems. Aerosp Med. 1966 Dec;37(12):1223–1228. [PubMed] [Google Scholar]
  14. Matsumoto H., Baba E., Ishikawa H. [Bacterial flora in the alimentary canal of dogs. 1. Normal flora in various parts of the intestinal tract]. Nihon Juigaku Zasshi. 1972 Oct;34(5):255–261. doi: 10.1292/jvms1939.34.255. [DOI] [PubMed] [Google Scholar]
  15. SMITH H. W. OBSERVATIONS ON THE FLORA OF THE ALIMENTARY TRACT OF ANIMALS AND FACTORS AFFECTING ITS COMPOSITION. J Pathol Bacteriol. 1965 Jan;89:95–122. [PubMed] [Google Scholar]
  16. Savage D. C., Dubos R. Alterations in the mouse cecum and its flora produced by antibacterial drugs. J Exp Med. 1968 Jul 1;128(1):97–110. doi: 10.1084/jem.128.1.97. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Taylor G. R., Henney M. R., Ellis W. L. Changes in the fungal autoflora of Apollo astronauts. Appl Microbiol. 1973 Nov;26(5):804–813. doi: 10.1128/am.26.5.804-813.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Yale C. E., Balish E. Blood and serum chemistry values of gnotobiotic beagles. Lab Anim Sci. 1976 Aug;26(4):633–639. [PubMed] [Google Scholar]
  19. Zaloguyev S. N., Utkina T. G., Shinkareva M. M. The microflora of the human integument during prolonged confinement. Life Sci Space Res. 1971;9:55–59. [PubMed] [Google Scholar]

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