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. 1977 Nov;34(5):512–517. doi: 10.1128/aem.34.5.512-517.1977

Determination of pools of tricarboxylic acid cycle and related acids in bacteria.

W H Siegel, T Donohue, R W Bernlohr
PMCID: PMC242692  PMID: 337897

Abstract

Methods for sampling, extracting, and quantitating the metabolic pools of organic acids from bacteria have been developed. The concentration of these metabolites was determined by a new gas chromatographic method that can quantitatively determine the levels of lactate, pyruvate, fumarate, succinate, malate, alpha-ketoglutarate, and citrate. Values obtained were confirmed by fluorimetric analyses of five of the individual acids. In Escherichia coli, pools range from about 1 to 5 mumol/g of dry weight, with a variation in replicate samples of 5 to 15%. Under similar conditions, these pools in Bacillus licheniformis are in the same range, although the pyruvic acid pool is significantly larger.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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