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. 1977 Dec;34(6):701–705. doi: 10.1128/aem.34.6.701-705.1977

Isolation of the etiological agent of primary amoebic meningoencephalitis from artifically heated waters.

A R Stevens, R L Tyndall, C C Coutant, E Willaert
PMCID: PMC242733  PMID: 596872

Abstract

To determine whether artificial heating of water by power plant discharges facilitates proliferation of the pathogenic free-living amoebae that cause primary amoebic meningoencephalitis, water samples (250 ml) were taken from discharges within 3,000 feet (ca. 914.4 m) of power plants and were processed for amoeba culture. Pathogenic Naegleria fowleri grew out of water samples from two of five lakes and rivers in Florida and from one of eight man-made lakes in Texas. Pathogenic N. fowleri did not grow from water samples taken from cooling towers and control lakes, the latter of which had no associated power plants. The identification of N. fowleri was confirmed by pathogenicity in mice and by indirect immunofluorescence analyses, by using a specific antiserum.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Carter R. F. Description of a Naegleria sp. isolated from two cases of primary amoebic meningo-encephalitis, and of the experimental pathological changes induced by it. J Pathol. 1970 Apr;100(4):217–244. doi: 10.1002/path.1711000402. [DOI] [PubMed] [Google Scholar]
  2. Carter R. F. Primary amoebic meningo-encephalitis: clinical, pathological and epidemiological features of six fatal cases. J Pathol Bacteriol. 1968 Jul;96(1):1–25. doi: 10.1002/path.1700960102. [DOI] [PubMed] [Google Scholar]
  3. De Jonckheere J., Van Dijck P., Van de Voorde H. The effect of thermal pollution on the distribution of Naegleria fowleri. J Hyg (Lond) 1975 Aug;75(1):7–13. doi: 10.1017/s0022172400047021. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. De Jonckheere J., van de Voorde H. Differences in destruction of cysts of pathogenic and nonpathogenic Naegleria and Acanthamoeba by chlorine. Appl Environ Microbiol. 1976 Feb;31(2):294–297. doi: 10.1128/aem.31.2.294-297.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Derreumaux A. L., Jadin J. B., Willaert E., Moret R. Action du chlore sur les amibes de l'eau. Ann Soc Belg Med Trop. 1974;54(4-5):415–428. [PubMed] [Google Scholar]
  6. Griffin J. L. Temperature tolerance of pathogenic and nonpathogenic free-living amoebas. Science. 1972 Nov 24;178(4063):869–870. doi: 10.1126/science.178.4063.869. [DOI] [PubMed] [Google Scholar]
  7. Martínez A. J., Sotelo-Avila C., Garcia-Tamayo J., Morón J. T., Willaert E., Stamm W. P. Meningoencephalitis due to Acanthamoeba SP. Pathogenesis and clinico-pathological study. Acta Neuropathol. 1977 Mar 31;37(3):183–191. doi: 10.1007/BF00686877. [DOI] [PubMed] [Google Scholar]
  8. Taylor P. W. Isolation and experimental infection of free-living amebae in freshwater fishes. J Parasitol. 1977 Apr;63(2):232–237. [PubMed] [Google Scholar]
  9. Wellings F. M., Lewis A. L., Amuso P. T., Chang S. L. Naegleria and water sports. Lancet. 1977 Jan 22;1(8004):199–200. doi: 10.1016/s0140-6736(77)91806-2. [DOI] [PubMed] [Google Scholar]
  10. Willaert E. Etude emmuno-taxonomique des genres Naegleria et Acanthamoeba (Protozoa: Amoebida) Acta Zool Pathol Antverp. 1976 Dec;(65):1–239. [PubMed] [Google Scholar]
  11. Willaert E. Isolement et culture in vitro des amibes du genre Naegleria. Ann Soc Belges Med Trop Parasitol Mycol. 1971;51(6):701–708. [PubMed] [Google Scholar]
  12. Willaert E., Stevens A. R. Isolation of pathogenic amoeba from thermal-discharge water. Lancet. 1976 Oct 2;2(7988):741–741. doi: 10.1016/s0140-6736(76)90035-0. [DOI] [PubMed] [Google Scholar]

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