Abstract
The neuraminidases of 3 human A2 virus strains—A2/Singapore/1/57, A2/England/76/66 and A2/Hong Kong/1/68—were compared immunologically with those of a number of influenza A virus strains of animal origin (14 avian, 2 equine and 1 porcine). The methods used were enzyme-inhibition tests and immuno-double-diffusion. In enzyme-inhibition tests, the neuraminidases of the A2/57 and A2/66 viruses cross-reacted with those of Turkey/Wisconsin/66, Turkey/Massachusetts/65 and Duck/Italy/574/66 viruses. The enzyme of the Hong Kong variant had a wider spectrum of cross-reactions than did those of the A2/57 and A2/66 viruses. No cross-reaction was detected with Turkey/Massachusetts/65 but the A2/Hong Kong enzyme showed major antigenic similarities with the enzymes of Turkey/Wisconsin/66 and Duck/Italy/574/66 and Duck/Germany/1998/68 viruses. Minor cross-reactions were also detected between the A2/Hong Kong enzyme and those of fowl plague virus, virus ”N”, Duck/England/56 and Swine/Cambridge/39.
The cross-reactions between the enzymes of A2/Hong Kong and Turkey/Wisconsin/66 were confirmed by immunoprecipitin tests. Those between A2/Hong Kong and fowl plague virus, virus ”N”, Duck/England/56 and Swine/Cambridge/39 could not be confirmed by immunoprecipitation.
The finding of antigenic similarities between the enzymes of human and avian influenza A viruses may be of importance in considering the origin of the new antigenic variants of influenza A virus which appear in man.
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- CROWLE A. J. A simplified micro double-diffusion agar precipitin technique. J Lab Clin Med. 1958 Nov;52(5):784–787. [PubMed] [Google Scholar]
- Easterday B., Laver W. G., Pereira H. G., Schild G. C. Antigenic composition of recombinant virus strains produced from human and avian influenza A viruses. J Gen Virol. 1969 Jul;5(1):83–91. doi: 10.1099/0022-1317-5-1-83. [DOI] [PubMed] [Google Scholar]
- Haukenes G., Harboe A., Mortensson-Egnund K. A uronic and sialic acid free chick allantoic mucopolysaccharide sulphate which combines with influenza virus hi-antibody to host material. 2. Chemical composition. Acta Pathol Microbiol Scand. 1966;66(4):510–518. doi: 10.1111/apm.1966.66.4.510. [DOI] [PubMed] [Google Scholar]
- Laver W. G., Webster R. G. The structure of influenza viruses. IV. Chemical studies of the host antigen. Virology. 1966 Sep;30(1):104–115. doi: 10.1016/s0042-6822(66)81014-0. [DOI] [PubMed] [Google Scholar]
- Pereira H. G. Influenza: antigenic spectrum. Prog Med Virol. 1969;11:46–79. [PubMed] [Google Scholar]
- Pereira H. G., Lang G., Olesiuk O. M., Snoeyenbos G. H., Roberts D. H., Easterday B. C. New antigenic variants of avian influenza A viruses. Bull World Health Organ. 1966;35(5):799–802. [PMC free article] [PubMed] [Google Scholar]
- Pereira H. G., Rinaldi A., Nardelli L. Antigenic variation among avian influenza A viruses. Bull World Health Organ. 1967;37(4):553–558. [PMC free article] [PubMed] [Google Scholar]
- Pereira H. G., Tumova B., Webster R. G. Antigenic relationship between influenza A viruses of human and avian origins. Nature. 1967 Aug 26;215(5104):982–983. doi: 10.1038/215982a0. [DOI] [PubMed] [Google Scholar]
- Pereira H. G., Tůmová B., Law V. G. Avian influenza A viruses. Bull World Health Organ. 1965;32(6):855–860. [PMC free article] [PubMed] [Google Scholar]
- Schild G. C., Pereira H. G. Characterization of the ribonucleoprotein and neuraminidase of influenza A viruses by immunodiffusion. J Gen Virol. 1969 Apr;4(3):355–363. doi: 10.1099/0022-1317-4-3-355. [DOI] [PubMed] [Google Scholar]
- Schild G. C., Pereira H. G., Schettler C. H. Neuraminidase in avin influenza A virus antigenically related to that of human A0 and A1 subtypes. Nature. 1969 Jun 28;222(5200):1299–1301. doi: 10.1038/2221299a0. [DOI] [PubMed] [Google Scholar]
- Seto J. T., Rott R. Functional significance of sialidose during influenza virus multiplication. Virology. 1966 Dec;30(4):731–737. doi: 10.1016/0042-6822(66)90178-4. [DOI] [PubMed] [Google Scholar]
- Tumova B., Pereira H. G. Genetic interaction between influenza A viruses of human and animal origin. Virology. 1965 Nov;27(3):253–261. doi: 10.1016/0042-6822(65)90104-2. [DOI] [PubMed] [Google Scholar]
- Tůmová B., Pereira H. G. Antigenic relationship between influenza A viruses of human and animal origin. Bull World Health Organ. 1968;38(3):415–420. [PMC free article] [PubMed] [Google Scholar]
- Webster R. G., Laver W. G., Kilbourne E. D. Reactions of antibodies with surface antigens of influenza virus. J Gen Virol. 1968 Dec;3(3):315–326. doi: 10.1099/0022-1317-3-3-315. [DOI] [PubMed] [Google Scholar]
- Webster R. G., Laver W. G. Preparation and properties of antibody directed specifically against the neuraminidase of influenza virus. J Immunol. 1967 Jul;99(1):49–55. [PubMed] [Google Scholar]
- Webster R. G., Pereira H. G. A common surface antigen in influenza viruses from human and avian sources. J Gen Virol. 1968 Sep;3(2):201–208. doi: 10.1099/0022-1317-3-2-201. [DOI] [PubMed] [Google Scholar]