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. 1971;44(5):593–598.

A comparative study of attenuated influenza viruses

A S Beare, H F Maassab, D A J Tyrrell, A N Slepuškin, T S Hall
PMCID: PMC2427844  PMID: 5316742

Abstract

Influenza A and influenza B viruses were adapted to growth at 25°C. When given to volunteer subjects, the viruses were attenuated but remained infective and antigenic. The minimum immunizing dose of an egg-adapted virus appeared to be 105.0 EID50. Cloning by plaque selection at 25°C gave seed cultures of relatively low infectivity titres. These titres were increased when necessary by passage at 33°C. No reversion to virulence was observed.

Viruses attenuated in the United Kingdom and the USA were compared in volunteer trials with vaccine strains that had already been used in the USSR for mass immunization. Results were broadly similar. Currently available methods of attenuation and work with temperature-sensitive mutants are reviewed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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