Skip to main content
PMC home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1978 Jul;36(1):36–42. doi: 10.1128/aem.36.1.36-42.1978

Improved medium for recovery and enumeration of Pseudomonas aeruginosa from water using membrane filters.

M H Brodsky, B W Ciebin
PMCID: PMC243030  PMID: 100052

Abstract

A modified mPA medium, designated mPA-C, was shown to recover Pseudomonas aeruginosa from a variety of water sources with results comparable to those with mPA-B and within the confidence limits of a most-probable-number technique. Enumeration of P. aeruginosa on mPA-C was possible after only 24 h of incubation at 41.5 degrees C, compared with 72 h of incubation required for mPA-B and 96 h of incubation for a presumptive most probable number.

Full text

PDF
41

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brodsky M. H., Ciebin B. W., Schiemann D. A. Simple bacterial preservation medium and its application to proficiency testing in water bacteriology. Appl Environ Microbiol. 1978 Mar;35(3):487–491. doi: 10.1128/aem.35.3.487-491.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brown M. R., Foster J. H. A simple diagnostic milk medium for Pseudomonas aeruginosa. J Clin Pathol. 1970 Mar;23(2):172–177. doi: 10.1136/jcp.23.2.172. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Drake C. H. Evaluation of culture media for the isolation and enumeration of Pseudomonas aeruginosa. Health Lab Sci. 1966 Jan;3(1):10–19. [PubMed] [Google Scholar]
  4. Dutka B. J., Kwan K. K. Confirmation of the single-step membrane filtration procedure for estimating Pseudomonas aeruginosa densities in water. Appl Environ Microbiol. 1977 Feb;33(2):240–245. doi: 10.1128/aem.33.2.240-245.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Eisenhart C., Wilson P. W. STATISTICAL METHODS AND CONTROL IN BACTERIOLOGY. Bacteriol Rev. 1943 Jun;7(2):57–137. doi: 10.1128/br.7.2.57-137.1943. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Factors influencing detection and enumeration of Pseudomonas aeruginosa by most-probable-number and membrane filtration techniques. Appl Microbiol. 1975 Dec;30(6):935–942. doi: 10.1128/am.30.6.935-942.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Highsmith A. K., Abshire R. L. Evaluation of a most-probable-number technique for the enumeration of Pseudomonas aeruginosa. Appl Microbiol. 1975 Oct;30(4):596–601. doi: 10.1128/am.30.4.596-601.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hoadley A. W., Ajello G., Masterson N. Preliminary studies of fluorescent pseudomonads capable of growth at 41 C in swimming pool waters. Appl Microbiol. 1975 Apr;29(4):527–531. doi: 10.1128/am.29.4.527-531.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hoadley A. W., Ajello G. Some characteristics of fluorescent pseudomonads isolated from surface waters and capable of growth at 41C. Can J Microbiol. 1972 Nov;18(11):1769–1773. doi: 10.1139/m72-274. [DOI] [PubMed] [Google Scholar]
  10. KING E. O., WARD M. K., RANEY D. E. Two simple media for the demonstration of pyocyanin and fluorescin. J Lab Clin Med. 1954 Aug;44(2):301–307. [PubMed] [Google Scholar]
  11. Levin M. A., Cabelli V. J. Membrane filter technique for enumeration of Pseudomonas aeruginosa. Appl Microbiol. 1972 Dec;24(6):864–870. doi: 10.1128/am.24.6.864-870.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Taylor W. I. Isolation of shigellae. I. Xylose lysine agars; new media for isolation of enteric pathogens. Am J Clin Pathol. 1965 Oct;44(4):471–475. [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES